Gene description for Prmt1
Gene name protein arginine methyltransferase 1
Gene symbol Prmt1
Other names/aliases Hrmt1l2
Species Rattus norvegicus
 Database cross references - Prmt1
ExoCarta ExoCarta_60421
Entrez Gene 60421
UniProt Q63009  
 Prmt1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Prmt1
Molecular Function
    protein-arginine N-methyltransferase activity GO:0016274 ISO
    poly(A) RNA binding GO:0044822 ISO
    [cytochrome c]-arginine N-methyltransferase activity GO:0016275 IDA
    histone methyltransferase activity GO:0042054 ISO
    identical protein binding GO:0042802 ISO
    protein-arginine omega-N asymmetric methyltransferase activity GO:0035242 IDA
    S-adenosylmethionine-dependent methyltransferase activity GO:0008757 TAS
    protein methyltransferase activity GO:0008276 ISO
    N-methyltransferase activity GO:0008170 ISO
    protein binding GO:0005515 IPI
    snoRNP binding GO:0030519 IDA
    protein-arginine omega-N monomethyltransferase activity GO:0035241 IDA
    histone methyltransferase activity (H4-R3 specific) GO:0044020 ISS
    histone-arginine N-methyltransferase activity GO:0008469 IDA
Biological Process
    peptidyl-arginine methylation, to asymmetrical-dimethyl arginine GO:0019919 IDA
    histone methylation GO:0016571 ISO
    neuron projection development GO:0031175 ISO
    in utero embryonic development GO:0001701 ISO
    peptidyl-arginine N-methylation GO:0035246 ISO
    peptidyl-arginine methylation GO:0018216 ISO
    histone H4-R3 methylation GO:0043985 ISS
    peptidyl-arginine omega-N-methylation GO:0035247 IDA
    regulation of transcription, DNA-templated GO:0006355 IBA
    protein methylation GO:0006479 ISO
    negative regulation of megakaryocyte differentiation GO:0045653 ISS
    liver regeneration GO:0097421 IEP
Subcellular Localization
    protein complex GO:0043234 IDA
    cytosol GO:0005829 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
 Experiment description of studies that identified Prmt1 in exosomes
1
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prmt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prmt1 is involved
PathwayEvidenceSource
RMTs methylate histone arginines IEA Reactome





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