Gene description for Hspa2
Gene name heat shock protein 2
Gene symbol Hspa2
Other names/aliases Hspt70
Hst70
Species Rattus norvegicus
 Database cross references - Hspa2
ExoCarta ExoCarta_60460
Vesiclepedia VP_60460
Entrez Gene 60460
UniProt P14659  
 Hspa2 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Hspa2
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    enzyme binding GO:0019899 ISO
    heat shock protein binding GO:0031072 IBA
    protein folding chaperone GO:0044183 IBA
    tau protein binding GO:0048156 IPI
    unfolded protein binding GO:0051082 ISO
    protein-folding chaperone binding GO:0051087 IPI
    glycolipid binding GO:0051861 ISO
    disordered domain specific binding GO:0097718 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 ISO
    male meiotic nuclear division GO:0007140 ISO
    male meiosis I GO:0007141 ISO
    spermatogenesis GO:0007283 ISO
    spermatogenesis GO:0007283 ISS
    spermatid development GO:0007286 ISO
    response to heat GO:0009408 ISS
    response to cold GO:0009409 ISS
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 ISO
    positive regulation of ATP-dependent activity GO:0032781 ISO
    protein refolding GO:0042026 IBA
    protein refolding GO:0042026 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    synaptonemal complex disassembly GO:0070194 ISO
    negative regulation of inclusion body assembly GO:0090084 ISO
    positive regulation of ATPase-coupled calcium transmembrane transporter activity GO:1901896 ISO
Subcellular Localization
    synaptonemal complex GO:0000795 ISO
    male germ cell nucleus GO:0001673 ISO
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    cell surface GO:0009986 ISO
    CatSper complex GO:0036128 ISO
    CatSper complex GO:0036128 ISS
    meiotic spindle GO:0072687 ISO
    meiotic spindle GO:0072687 ISS
 Experiment description of studies that identified Hspa2 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hspa2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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