Gene description for Hspa2
Gene name heat shock protein 2
Gene symbol Hspa2
Other names/aliases Hspt70
Hst70
Species Rattus norvegicus
 Database cross references - Hspa2
ExoCarta ExoCarta_60460
Entrez Gene 60460
UniProt P14659  
 Hspa2 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Hspa2
Molecular Function
    glycolipid binding GO:0051861 ISO
    ATP binding GO:0005524 IEA
    unfolded protein binding GO:0051082 ISO
    enzyme binding GO:0019899 ISO
Biological Process
    negative regulation of inclusion body assembly GO:0090084 ISO
    positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle GO:0031662 ISO
    spermatid development GO:0007286 ISO
    positive regulation of protein phosphorylation GO:0001934 ISO
    multicellular organismal development GO:0007275 IEA
    male meiosis I GO:0007141 ISO
    synaptonemal complex disassembly GO:0070194 ISO
    protein refolding GO:0042026 ISO
    male meiosis GO:0007140 ISO
Subcellular Localization
    CatSper complex GO:0036128 ISS
    membrane GO:0016020 ISO
    myelin sheath GO:0043209 ISO
    cytosol GO:0005829 ISO
    blood microparticle GO:0072562 ISO
    synaptonemal complex GO:0000795 ISO
    nucleus GO:0005634 ISO
    cell surface GO:0009986 ISO
    mitochondrion GO:0005739 ISO
    extracellular exosome GO:0070062 ISO
    male germ cell nucleus GO:0001673 ISO
 Experiment description of studies that identified Hspa2 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hspa2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hspa2 is involved
PathwayEvidenceSource
Meiotic synapsis IEA Reactome





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