Gene description for Wtap
Gene name Wilms tumour 1-associating protein
Gene symbol Wtap
Other names/aliases 2810408K05Rik
9430038B09Rik
Species Mus musculus
 Database cross references - Wtap
ExoCarta ExoCarta_60532
Vesiclepedia VP_60532
Entrez Gene 60532
UniProt Q9ER69  
 Wtap identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wtap
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 ISO
Biological Process
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IBA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISO
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISS
    mRNA processing GO:0006397 ISO
    mRNA processing GO:0006397 ISS
    RNA splicing GO:0008380 IEA
    mRNA modification GO:0016556 IBA
    mRNA modification GO:0016556 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    nuclear membrane GO:0031965 ISO
    RNA N6-methyladenosine methyltransferase complex GO:0036396 IDA
    RNA N6-methyladenosine methyltransferase complex GO:0036396 ISO
 Experiment description of studies that identified Wtap in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wtap
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cebpa  
Affinity Capture-MS Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Atg16l1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Wtap is involved
PathwayEvidenceSource
Metabolism of RNA IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome





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