Gene description for Napsa
Gene name napsin A aspartic peptidase
Gene symbol Napsa
Other names/aliases Kdap
Species Rattus norvegicus
 Database cross references - Napsa
ExoCarta ExoCarta_60575
Entrez Gene 60575
 Napsa identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Napsa
Molecular Function
    peptidase activity GO:0008233 ISO
    aspartic-type endopeptidase activity GO:0004190 IBA
    endopeptidase activity GO:0004175 ISO
Biological Process
    proteolysis GO:0006508 IBA
    membrane protein proteolysis GO:0033619 ISO
    protein catabolic process GO:0030163 IBA
    surfactant homeostasis GO:0043129 ISO
Subcellular Localization
    extracellular space GO:0005615 ISO
    lysosome GO:0005764 ISO
    extracellular exosome GO:0070062 ISO
    alveolar lamellar body GO:0097208 ISO
 Experiment description of studies that identified Napsa in exosomes
1
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Napsa
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Napsa is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here