ExoCarta: Gene summary

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Gene description for Ppp2r2b
Gene name	protein phosphatase 2, regulatory subunit B, beta
Gene symbol	Ppp2r2b
Other names/aliases	Pppr2b2
Species	Rattus norvegicus
Database cross references - Ppp2r2b
ExoCarta	ExoCarta_60660
Entrez Gene	60660
UniProt	P36877
Ppp2r2b identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Ppp2r2b

Molecular Function
	protein complex binding	GO:0032403	IDA
	protein phosphatase type 2A regulator activity	GO:0008601	IEA
	protein binding	GO:0005515	IPI
Biological Process
	regulation of protein phosphatase type 2A activity	GO:0034047	IDA
	protein targeting to mitochondrion	GO:0006626	IMP
	signal transduction	GO:0007165	IEA
	positive regulation of neuron apoptotic process	GO:0043525	IDA
	neuron differentiation	GO:0030182	IEP
	mitochondrial fission	GO:0000266	IDA
	brain development	GO:0007420	IEP
	mitochondrial fragmentation involved in apoptotic process	GO:0043653	IMP
	spermatid development	GO:0007286	IEP
Subcellular Localization
	mitochondrial outer membrane	GO:0005741	IEA
	protein phosphatase type 2A complex	GO:0000159	IDA
	membrane	GO:0016020	IDA
	cytoskeleton	GO:0005856	IDA
	cytosol	GO:0005829	IDA
	cytoplasm	GO:0005737	IDA
	mitochondrion	GO:0005739	IDA

Experiment description of studies that identified Ppp2r2b in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting