Gene description for Gpd1
Gene name glycerol-3-phosphate dehydrogenase 1 (soluble)
Gene symbol Gpd1
Other names/aliases GPDH
Gpd3
Species Rattus norvegicus
 Database cross references - Gpd1
ExoCarta ExoCarta_60666
Vesiclepedia VP_60666
Entrez Gene 60666
UniProt O35077  
 Gpd1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Gpd1
Molecular Function
    glycerol-3-phosphate dehydrogenase (quinone) activity GO:0004368 IDA
    protein homodimerization activity GO:0042803 IEA
    NAD binding GO:0051287 IDA
    NAD binding GO:0051287 IEA
    glycerol-3-phosphate dehydrogenase (NAD+) activity GO:0141152 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    glycerol-3-phosphate metabolic process GO:0006072 IBA
    glycerol-3-phosphate metabolic process GO:0006072 IDA
    glycerol-3-phosphate metabolic process GO:0006072 ISO
    gluconeogenesis GO:0006094 ISO
    NADH oxidation GO:0006116 IBA
    NADH oxidation GO:0006116 IDA
    NADH oxidation GO:0006116 IEA
    NADH oxidation GO:0006116 ISO
    glycerophosphate shuttle GO:0006127 ISO
    glycerophosphate shuttle GO:0006127 ISO
    NADH metabolic process GO:0006734 IDA
    positive regulation of glycolytic process GO:0045821 ISO
    glycerol-3-phosphate catabolic process GO:0046168 IEA
    glycerolipid metabolic process GO:0046486 IDA
    cellular response to cAMP GO:0071320 ISO
    cellular response to tumor necrosis factor GO:0071356 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Gpd1 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Gpd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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