Gene description for Gpd1
Gene name glycerol-3-phosphate dehydrogenase 1 (soluble)
Gene symbol Gpd1
Other names/aliases GPDH
Gpd3
Species Rattus norvegicus
 Database cross references - Gpd1
ExoCarta ExoCarta_60666
Entrez Gene 60666
UniProt O35077  
 Gpd1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Gpd1
Molecular Function
    glycerol-3-phosphate dehydrogenase activity GO:0004368 IDA
    NAD binding GO:0051287 IDA
    protein homodimerization activity GO:0042803 IEA
    glycerol-3-phosphate dehydrogenase [NAD+] activity GO:0004367 TAS
Biological Process
    NADH oxidation GO:0006116 IDA
    glycerolipid metabolic process GO:0046486 IDA
    NADH metabolic process GO:0006734 IDA
    cellular response to tumor necrosis factor GO:0071356 ISO
    glycerophosphate shuttle GO:0006127 ISO
    oxidation-reduction process GO:0055114 ISO
    gluconeogenesis GO:0006094 ISO
    cellular response to cAMP GO:0071320 ISO
    glycerol-3-phosphate catabolic process GO:0046168 IEA
    glycerol-3-phosphate metabolic process GO:0006072 ISO
    positive regulation of glycolytic process GO:0045821 ISO
    carbohydrate metabolic process GO:0005975 IDA
Subcellular Localization
    glycerol-3-phosphate dehydrogenase complex GO:0009331 IEA
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 IDA
    intracellular GO:0005622 ISO
    cell GO:0005623 ISO
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Gpd1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Gpd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gpd1 is involved
PathwayEvidenceSource
Synthesis of PA IEA Reactome
Triglyceride Biosynthesis IEA Reactome





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