Gene description for Cyb5a
Gene name cytochrome b5 type A (microsomal)
Gene symbol Cyb5a
Other names/aliases Cyb5
Species Rattus norvegicus
 Database cross references - Cyb5a
ExoCarta ExoCarta_64001
Entrez Gene 64001
UniProt P00173  
 Cyb5a identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Cyb5a
Molecular Function
    heme binding GO:0020037 IEA
    electron carrier activity GO:0009055 TAS
    metal ion binding GO:0046872 IEA
    molecular_function GO:0003674 ND
    enzyme binding GO:0019899 ISO
Biological Process
    oxidation-reduction process GO:0055114 IEA
    response to cadmium ion GO:0046686 IEP
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    endoplasmic reticulum GO:0005783 IDA
    mitochondrion GO:0005739 ISO
    membrane GO:0016020 ISO
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum membrane GO:0005789 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified Cyb5a in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Cyb5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cyb5a is involved
PathwayEvidenceSource
Vitamin C (ascorbate) metabolism IEA Reactome





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