ExoCarta: Gene summary

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Gene description for Slc22a4
Gene name	solute carrier family 22 (organic cation/zwitterion transporter), member 4
Gene symbol	Slc22a4
Other names/aliases	Octn1
Species	Rattus norvegicus
Database cross references - Slc22a4
ExoCarta	ExoCarta_64037
Entrez Gene	64037
UniProt	Q9R141
Slc22a4 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Slc22a4

Molecular Function
	cation:cation antiporter activity	GO:0015491	ISO
	quaternary ammonium group transmembrane transporter activity	GO:0015651	ISO
	symporter activity	GO:0015293	IEA
	carnitine transmembrane transporter activity	GO:0015226	ISO
	organic cation transmembrane transporter activity	GO:0015101	IDA
	PDZ domain binding	GO:0030165	ISO
	ATP binding	GO:0005524	IEA
Biological Process
	carnitine transmembrane transport	GO:1902603	ISO
	quaternary ammonium group transport	GO:0015697	ISO
	carnitine transport	GO:0015879	ISO
	modified amino acid transport	GO:0072337	ISO
	organic cation transport	GO:0015695	ISO
	sodium ion transport	GO:0006814	IEA
	ion transmembrane transport	GO:0034220	IEA
	carnitine metabolic process	GO:0009437	ISO
	triglyceride metabolic process	GO:0006641	ISO
Subcellular Localization
	apical plasma membrane	GO:0016324	ISO
	mitochondrion	GO:0005739	ISO
	integral component of membrane	GO:0016021	IEA

Experiment description of studies that identified Slc22a4 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ HSC70\|HSP90\|TSG101	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting