Gene description for Sptan1
Gene name spectrin, alpha, non-erythrocytic 1
Gene symbol Sptan1
Other names/aliases A2a
IPF
Spna2
Species Rattus norvegicus
 Database cross references - Sptan1
ExoCarta ExoCarta_64159
Entrez Gene 64159
UniProt P16086  
 Sptan1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Sptan1
Molecular Function
    protein heterodimerization activity GO:0046982 ISO
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 IEA
    calmodulin binding GO:0005516 IEA
    protein N-terminus binding GO:0047485 IPI
    actin binding GO:0003779 IEA
    protein complex binding GO:0032403 IPI
    syntaxin binding GO:0019905 IPI
    spectrin binding GO:0030507 ISO
Biological Process
    actin cytoskeleton reorganization GO:0031532 IMP
    actin filament capping GO:0051693 IEA
Subcellular Localization
    microtubule cytoskeleton GO:0015630 ISO
    plasma membrane GO:0005886 IDA
    spectrin GO:0008091 TAS
    intracellular membrane-bounded organelle GO:0043231 ISO
    fascia adherens GO:0005916 ISO
    Z disc GO:0030018 ISO
    cuticular plate GO:0032437 ISO
    paranode region of axon GO:0033270 ISO
    myelin sheath GO:0043209 ISO
    cytosol GO:0005829 TAS
    lateral plasma membrane GO:0016328 ISO
    membrane GO:0016020 ISO
    cortical cytoskeleton GO:0030863 ISO
    extracellular vesicle GO:1903561 ISO
    extracellular exosome GO:0070062 ISO
    protein complex GO:0043234 IDA
 Experiment description of studies that identified Sptan1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Sptan1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sptan1 is involved
PathwayEvidenceSource
Nephrin interactions IEA Reactome





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