Gene description for Arl1
Gene name ADP-ribosylation factor-like 1
Gene symbol Arl1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arl1
ExoCarta ExoCarta_64187
Vesiclepedia VP_64187
Entrez Gene 64187
UniProt P61212  
 Arl1 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Arl1
Molecular Function
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    enzyme activator activity GO:0008047 ISO
    enzyme activator activity GO:0008047 ISS
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    metal ion binding GO:0046872 IEA
    phospholipase D activator activity GO:1990583 IEA
    phospholipase D activator activity GO:1990583 ISO
    phospholipase D activator activity GO:1990583 ISS
Biological Process
    intracellular protein transport GO:0006886 IBA
    Golgi organization GO:0007030 IMP
    toxin metabolic process GO:0009404 IEA
    toxin metabolic process GO:0009404 ISO
    toxin metabolic process GO:0009404 ISS
    vesicle-mediated transport GO:0016192 IBA
    protein localization to Golgi apparatus GO:0034067 IEA
    protein localization to Golgi apparatus GO:0034067 ISO
    protein localization to Golgi apparatus GO:0034067 ISS
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    retrograde transport, endosome to Golgi GO:0042147 ISS
    Golgi vesicle transport GO:0048193 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    trans-Golgi network GO:0005802 IDA
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    cytosol GO:0005829 IEA
 Experiment description of studies that identified Arl1 in sEVs
1
Experiment ID 92
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Arl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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