Gene description for Pafah1b2
Gene name platelet-activating factor acetylhydrolase 1b, catalytic subunit 2
Gene symbol Pafah1b2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Pafah1b2
ExoCarta ExoCarta_64189
Vesiclepedia VP_64189
Entrez Gene 64189
UniProt O35264  
 Pafah1b2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Pafah1b2
Molecular Function
    1-alkyl-2-acetylglycerophosphocholine esterase activity GO:0003847 IDA
    1-alkyl-2-acetylglycerophosphocholine esterase activity GO:0003847 ISS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 ISS
    protein-containing complex binding GO:0044877 IPI
    protein heterodimerization activity GO:0046982 ISS
    platelet-activating factor acetyltransferase activity GO:0047179 IBA
    platelet-activating factor acetyltransferase activity GO:0047179 IDA
    platelet-activating factor acetyltransferase activity GO:0047179 TAS
Biological Process
    spermatogenesis GO:0007283 ISO
    spermatogenesis GO:0007283 ISO
    spermatogenesis GO:0007283 ISS
    lipid catabolic process GO:0016042 IEA
    positive regulation of macroautophagy GO:0016239 ISO
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    1-alkyl-2-acetylglycerophosphocholine esterase complex GO:0008247 IDA
    1-alkyl-2-acetylglycerophosphocholine esterase complex GO:0008247 ISS
 Experiment description of studies that identified Pafah1b2 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pafah1b2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here