Gene description for Bcat2
Gene name branched chain amino acid transaminase 2, mitochondrial
Gene symbol Bcat2
Other names/aliases BCT2
Bcatm
mBcat
Species Rattus norvegicus
 Database cross references - Bcat2
ExoCarta ExoCarta_64203
Entrez Gene 64203
UniProt O35854  
 Bcat2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Bcat2
Molecular Function
    branched-chain-amino-acid transaminase activity GO:0004084 ISO
    L-isoleucine transaminase activity GO:0052656 IEA
    L-leucine transaminase activity GO:0052654 IEA
    L-valine transaminase activity GO:0052655 IEA
Biological Process
    regulation of hormone levels GO:0010817 ISO
    branched-chain amino acid metabolic process GO:0009081 TAS
    lactation GO:0007595 IEP
    leucine metabolic process GO:0006551 ISO
    branched-chain amino acid catabolic process GO:0009083 ISO
    isoleucine catabolic process GO:0006550 ISO
    leucine biosynthetic process GO:0009098 IDA
    branched-chain amino acid biosynthetic process GO:0009082 IEA
    valine metabolic process GO:0006573 ISO
    isoleucine metabolic process GO:0006549 ISO
Subcellular Localization
    mitochondrion GO:0005739 ISO
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified Bcat2 in exosomes
1
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Bcat2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Bcat2 is involved
No pathways found





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