Gene description for Bcat2
Gene name branched chain amino acid transaminase 2, mitochondrial
Gene symbol Bcat2
Other names/aliases BCT2
Bcatm
mBcat
Species Rattus norvegicus
 Database cross references - Bcat2
ExoCarta ExoCarta_64203
Vesiclepedia VP_64203
Entrez Gene 64203
UniProt O35854  
 Bcat2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Bcat2
Molecular Function
    branched-chain-amino-acid transaminase activity GO:0004084 IBA
    branched-chain-amino-acid transaminase activity GO:0004084 IDA
    branched-chain-amino-acid transaminase activity GO:0004084 IEA
    branched-chain-amino-acid transaminase activity GO:0004084 ISO
    L-leucine-2-oxoglutarate transaminase activity GO:0052654 IDA
    L-leucine-2-oxoglutarate transaminase activity GO:0052654 IEA
    L-leucine-2-oxoglutarate transaminase activity GO:0052654 ISO
    L-valine-2-oxoglutarate transaminase activity GO:0052655 IDA
    L-valine-2-oxoglutarate transaminase activity GO:0052655 IEA
    L-valine-2-oxoglutarate transaminase activity GO:0052655 ISO
    L-isoleucine-2-oxoglutarate transaminase activity GO:0052656 IDA
    L-isoleucine-2-oxoglutarate transaminase activity GO:0052656 IEA
    L-isoleucine-2-oxoglutarate transaminase activity GO:0052656 ISO
Biological Process
    isoleucine metabolic process GO:0006549 ISO
    isoleucine catabolic process GO:0006550 IDA
    isoleucine catabolic process GO:0006550 IEA
    isoleucine catabolic process GO:0006550 ISO
    L-leucine metabolic process GO:0006551 IEA
    L-leucine metabolic process GO:0006551 ISO
    valine metabolic process GO:0006573 IEA
    valine metabolic process GO:0006573 ISO
    lipid metabolic process GO:0006629 IEA
    lactation GO:0007595 IEP
    amino acid biosynthetic process GO:0008652 IEA
    branched-chain amino acid metabolic process GO:0009081 ISO
    branched-chain amino acid metabolic process GO:0009081 TAS
    branched-chain amino acid biosynthetic process GO:0009082 IEA
    branched-chain amino acid catabolic process GO:0009083 IDA
    branched-chain amino acid catabolic process GO:0009083 ISO
    L-leucine biosynthetic process GO:0009098 IBA
    L-leucine biosynthetic process GO:0009098 IDA
    L-valine biosynthetic process GO:0009099 IBA
    regulation of hormone levels GO:0010817 IEA
    regulation of hormone levels GO:0010817 ISO
    cellular response to leukemia inhibitory factor GO:1990830 IEA
    cellular response to leukemia inhibitory factor GO:1990830 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Bcat2 in sEVs
1
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Bcat2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Bcat2 is involved
PathwayEvidenceSource
Branched-chain amino acid catabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome





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