Gene description for Mthfd1
Gene name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Gene symbol Mthfd1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Mthfd1
ExoCarta ExoCarta_64300
Entrez Gene 64300
UniProt P27653  
 Mthfd1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Mthfd1
Molecular Function
    formate-tetrahydrofolate ligase activity GO:0004329 TAS
    methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity GO:0004486 TAS
    ATP binding GO:0005524 IEA
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IEA
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 ISO
    hydrolase activity GO:0016787 IEA
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 TAS
Biological Process
    somite development GO:0061053 ISO
    transsulfuration GO:0019346 ISO
    serine family amino acid biosynthetic process GO:0009070 ISO
    purine nucleotide biosynthetic process GO:0006164 ISO
    oxidation-reduction process GO:0055114 IEA
    nucleotide metabolic process GO:0009117 IEP
    methionine metabolic process GO:0006555 ISO
    10-formyltetrahydrofolate biosynthetic process GO:0009257 ISO
    neural tube closure GO:0001843 ISO
    tetrahydrofolate interconversion GO:0035999 ISO
    folic acid-containing compound biosynthetic process GO:0009396 IEA
    tetrahydrofolate metabolic process GO:0046653 TAS
    histidine biosynthetic process GO:0000105 IEA
    one-carbon metabolic process GO:0006730 ISO
    methionine biosynthetic process GO:0009086 ISO
    heart development GO:0007507 ISO
    serine family amino acid metabolic process GO:0009069 ISO
    neutrophil homeostasis GO:0001780 ISO
Subcellular Localization
    mitochondrion GO:0005739 ISO
    cytoplasm GO:0005737 TAS
    membrane GO:0016020 ISO
    cytosol GO:0005829 IBA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Mthfd1 in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 101
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Mthfd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mthfd1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here