Gene description for Pfn1
Gene name profilin 1
Gene symbol Pfn1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Pfn1
ExoCarta ExoCarta_64303
Vesiclepedia VP_64303
Entrez Gene 64303
UniProt P62963  
 Pfn1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Pfn1
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IEA
    adenyl-nucleotide exchange factor activity GO:0000774 ISO
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    actin binding GO:0003779 IBA
    actin binding GO:0003779 ISO
    actin binding GO:0003779 TAS
    actin monomer binding GO:0003785 IEA
    actin monomer binding GO:0003785 ISO
    signaling receptor binding GO:0005102 IDA
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISO
    small GTPase binding GO:0031267 ISO
    proline-rich region binding GO:0070064 IEA
    proline-rich region binding GO:0070064 ISO
Biological Process
    neural tube closure GO:0001843 ISO
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    actin polymerization or depolymerization GO:0008154 TAS
    positive regulation of epithelial cell migration GO:0010634 IEA
    positive regulation of epithelial cell migration GO:0010634 ISO
    actin cytoskeleton organization GO:0030036 IEA
    regulation of actin filament polymerization GO:0030833 IBA
    negative regulation of actin filament polymerization GO:0030837 IEA
    negative regulation of actin filament polymerization GO:0030837 ISO
    positive regulation of actin filament polymerization GO:0030838 IEA
    positive regulation of actin filament polymerization GO:0030838 ISO
    negative regulation of actin filament bundle assembly GO:0032232 ISO
    positive regulation of actin filament bundle assembly GO:0032233 IBA
    positive regulation of ATP-dependent activity GO:0032781 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of viral transcription GO:0050434 IMP
    modulation of chemical synaptic transmission GO:0050804 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    positive regulation of DNA metabolic process GO:0051054 IDA
    positive regulation of stress fiber assembly GO:0051496 IMP
    negative regulation of stress fiber assembly GO:0051497 IEA
    negative regulation of stress fiber assembly GO:0051497 ISO
    synapse maturation GO:0060074 ISO
    cellular response to growth factor stimulus GO:0071363 IEP
    modification of postsynaptic actin cytoskeleton GO:0098885 ISO
    positive regulation of ruffle assembly GO:1900029 IEA
    positive regulation of ruffle assembly GO:1900029 ISO
    response to tetrachloromethane GO:1904772 IEP
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    cell cortex GO:0005938 IEA
    cell cortex GO:0005938 ISO
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    parallel fiber to Purkinje cell synapse GO:0098688 IDA
    presynapse GO:0098793 IDA
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Pfn1 in exosomes
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Pfn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gja1  
Affinity Capture-MS Rattus norvegicus
2 Dao  
Affinity Capture-MS Rattus norvegicus
3 Gphn  
Reconstituted Complex Rattus norvegicus
4 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
5 Glrb  
Affinity Capture-Western Rattus norvegicus
6 Sumo3  
Affinity Capture-MS Rattus norvegicus
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