Gene description for Gstm5
Gene name glutathione S-transferase, mu 5
Gene symbol Gstm5
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gstm5
ExoCarta ExoCarta_64352
Vesiclepedia VP_64352
Entrez Gene 64352
UniProt Q9Z1B2  
 Gstm5 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gstm5
Molecular Function
    glutathione transferase activity GO:0004364 IBA
    glutathione transferase activity GO:0004364 IDA
    glutathione transferase activity GO:0004364 IEA
    glutathione transferase activity GO:0004364 ISO
    enzyme binding GO:0019899 ISO
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    glutathione binding GO:0043295 ISO
Biological Process
    glutathione metabolic process GO:0006749 IBA
    glutathione metabolic process GO:0006749 ISO
    glutathione metabolic process GO:0006749 ISS
    biological_process GO:0008150 ND
    nitrobenzene metabolic process GO:0018916 ISO
    xenobiotic catabolic process GO:0042178 ISO
    response to estrogen GO:0043627 ISO
    cellular detoxification of nitrogen compound GO:0070458 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    sperm fibrous sheath GO:0035686 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
 Experiment description of studies that identified Gstm5 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gstm5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Gstm5 is involved
PathwayEvidenceSource
Biological oxidations IEA Reactome
Glutathione conjugation IEA Reactome
Metabolism IEA Reactome
Phase II - Conjugation of compounds IEA Reactome





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