Gene description for Cdc42
Gene name cell division cycle 42
Gene symbol Cdc42
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Cdc42
ExoCarta ExoCarta_64465
Vesiclepedia VP_64465
Entrez Gene 64465
UniProt Q8CFN2  
 Cdc42 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Cdc42
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 TAS
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 ISO
    GTP-dependent protein binding GO:0030742 ISO
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IPI
    thioesterase binding GO:0031996 ISO
    GBD domain binding GO:0032427 ISO
    apolipoprotein A-I receptor binding GO:0034191 ISO
    identical protein binding GO:0042802 ISO
    ubiquitin protein ligase activity GO:0061630 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    sprouting angiogenesis GO:0002040 IMP
    heart process GO:0003015 ISO
    cardiac conduction system development GO:0003161 ISO
    cardiac neural crest cell migration involved in outflow tract morphogenesis GO:0003253 ISO
    protein phosphorylation GO:0006468 ISO
    endocytosis GO:0006897 IBA
    endocytosis GO:0006897 ISO
    phagocytosis, engulfment GO:0006911 ISO
    phagocytosis, engulfment GO:0006911 ISS
    actin filament organization GO:0007015 IBA
    actin filament organization GO:0007015 IMP
    actin filament organization GO:0007015 ISO
    actin filament organization GO:0007015 ISS
    Golgi organization GO:0007030 IMP
    regulation of mitotic nuclear division GO:0007088 ISO
    nuclear migration GO:0007097 ISO
    establishment or maintenance of cell polarity GO:0007163 IEA
    integrin-mediated signaling pathway GO:0007229 ISO
    small GTPase-mediated signal transduction GO:0007264 IEA
    protein localization GO:0008104 ISO
    anatomical structure morphogenesis GO:0009653 IEA
    response to glucose GO:0009749 TAS
    regulation of lamellipodium assembly GO:0010591 ISO
    positive regulation of lamellipodium assembly GO:0010592 ISO
    regulation of exocytosis GO:0017157 TAS
    substantia nigra development GO:0021762 ISO
    establishment of cell polarity GO:0030010 IBA
    establishment of cell polarity GO:0030010 TAS
    actin cytoskeleton organization GO:0030036 ISO
    positive regulation of cell growth GO:0030307 ISO
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of pseudopodium assembly GO:0031274 ISO
    negative regulation of protein-containing complex assembly GO:0031333 ISO
    positive regulation of cytokinesis GO:0032467 ISO
    positive regulation of cytokinesis GO:0032467 ISS
    Cdc42 protein signal transduction GO:0032488 IBA
    regulation of actin cytoskeleton organization GO:0032956 ISO
    cell junction assembly GO:0034329 ISO
    cell junction assembly GO:0034329 ISS
    adherens junction organization GO:0034332 ISO
    embryonic heart tube development GO:0035050 ISO
    establishment or maintenance of apical/basal cell polarity GO:0035088 ISO
    dendritic cell migration GO:0036336 ISO
    neuropilin signaling pathway GO:0038189 ISO
    positive regulation of catalytic activity GO:0043085 IMP
    positive regulation of MAPK cascade GO:0043410 ISO
    positive regulation of neuron apoptotic process GO:0043525 IMP
    modulation by host of viral process GO:0044788 IEA
    establishment of epithelial cell apical/basal polarity GO:0045198 ISO
    establishment of epithelial cell apical/basal polarity GO:0045198 ISS
    positive regulation of DNA replication GO:0045740 IMP
    positive regulation of JNK cascade GO:0046330 IDA
    filopodium assembly GO:0046847 ISO
    positive regulation of pinocytosis GO:0048549 ISO
    positive regulation of pinocytosis GO:0048549 ISS
    neuron fate determination GO:0048664 ISO
    positive regulation of cellular component organization GO:0051130 IEA
    regulation of filopodium assembly GO:0051489 IBA
    regulation of filopodium assembly GO:0051489 ISO
    regulation of filopodium assembly GO:0051489 ISS
    positive regulation of filopodium assembly GO:0051491 ISO
    positive regulation of filopodium assembly GO:0051491 ISS
    regulation of stress fiber assembly GO:0051492 ISO
    positive regulation of stress fiber assembly GO:0051496 ISO
    nucleus localization GO:0051647 ISO
    establishment of localization in cell GO:0051649 ISO
    establishment of Golgi localization GO:0051683 IMP
    positive regulation of synapse structural plasticity GO:0051835 IMP
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISO
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 ISO
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 ISS
    heart contraction GO:0060047 ISO
    positive regulation of epithelial cell proliferation involved in lung morphogenesis GO:0060501 ISO
    submandibular salivary gland formation GO:0060661 IEP
    dendritic spine morphogenesis GO:0060997 ISO
    dendritic spine morphogenesis GO:0060997 ISS
    cellular response to type II interferon GO:0071346 ISO
    organelle transport along microtubule GO:0072384 IMP
    endothelin receptor signaling pathway involved in heart process GO:0086101 ISO
    actin filament branching GO:0090135 IMP
    positive regulation of intracellular protein transport GO:0090316 IMP
    regulation of modification of postsynaptic structure GO:0099159 IDA
    regulation of modification of postsynaptic structure GO:0099159 IMP
    regulation of postsynapse organization GO:0099175 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    storage vacuole GO:0000322 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 ISO
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    Golgi transport complex GO:0017119 ISO
    secretory granule GO:0030141 IDA
    filopodium GO:0030175 ISO
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    cell leading edge GO:0031252 ISO
    leading edge membrane GO:0031256 ISO
    lamellipodium membrane GO:0031258 IEA
    protein-containing complex GO:0032991 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    cell projection GO:0042995 ISO
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 IBA
    apical part of cell GO:0045177 ISO
    phagocytic vesicle GO:0045335 ISO
    spindle midzone GO:0051233 ISO
    spindle midzone GO:0051233 ISS
    cell periphery GO:0071944 ISO
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Cdc42 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Cdc42
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pak2  
Reconstituted Complex Rattus norvegicus
Co-purification Rattus norvegicus
2 Pak1  
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
3 Tnk2  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Cdc42 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
CD28 co-stimulation IEA Reactome
CD28 dependent Vav1 pathway IEA Reactome
CDC42 GTPase cycle IEA Reactome
Costimulation by the CD28 family IEA Reactome
DCC mediated attractive signaling IEA Reactome
Developmental Biology IEA Reactome
EGFR downregulation IEA Reactome
EPH-Ephrin signaling IEA Reactome
EPHB-mediated forward signaling IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
G beta:gamma signalling through CDC42 IEA Reactome
G-protein beta:gamma signalling IEA Reactome
GPCR downstream signalling IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Myogenesis IEA Reactome
Nervous system development IEA Reactome
Netrin-1 signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
RAC1 GTPase cycle IEA Reactome
RAC2 GTPase cycle IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
RHO GTPases activate KTN1 IEA Reactome
RHO GTPases activate PAKs IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RHOG GTPase cycle IEA Reactome
RHOQ GTPase cycle IEA Reactome
RHOU GTPase cycle IEA Reactome
RHOV GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome





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