Gene description for Eif2b1
Gene name eukaryotic translation initiation factor 2B, subunit 1 alpha
Gene symbol Eif2b1
Other names/aliases eIF-2a
Species Rattus norvegicus
 Database cross references - Eif2b1
ExoCarta ExoCarta_64514
Entrez Gene 64514
UniProt Q64270  
 Eif2b1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Eif2b1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    GTP binding GO:0005525 IMP
    GDP binding GO:0019003 IDA
    enzyme regulator activity GO:0030234 IMP
    S-methyl-5-thioribose-1-phosphate isomerase activity GO:0046523 IBA
    translation initiation factor activity GO:0003743 TAS
Biological Process
    oligodendrocyte development GO:0014003 ISS
    negative regulation of translational initiation in response to stress GO:0032057 IMP
    response to heat GO:0009408 IDA
    L-methionine biosynthetic process from methylthioadenosine GO:0019509 IBA
    regulation of translation GO:0006417 IMP
    translation GO:0006412 TAS
    response to glucose GO:0009749 IDA
    translational initiation GO:0006413 ISS
    positive regulation of GTPase activity GO:0043547 ISO
    response to peptide hormone GO:0043434 IDA
Subcellular Localization
    cytoplasm GO:0005737 ISS
    eukaryotic translation initiation factor 2B complex GO:0005851 ISS
    eukaryotic translation initiation factor 2 complex GO:0005850 TAS
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Eif2b1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eif2b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Eif2b1 is involved
PathwayEvidenceSource
Recycling of eIF2:GDP IEA Reactome





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