Gene description for Arl3
Gene name ADP-ribosylation factor-like 3
Gene symbol Arl3
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arl3
ExoCarta ExoCarta_64664
Vesiclepedia VP_64664
Entrez Gene 64664
UniProt P37996  
 Arl3 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Arl3
Molecular Function
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    microtubule binding GO:0008017 ISS
    GDP binding GO:0019003 IDA
    GDP binding GO:0019003 IEA
    GDP binding GO:0019003 ISO
Biological Process
    mitotic cytokinesis GO:0000281 IEA
    mitotic cytokinesis GO:0000281 ISO
    mitotic cytokinesis GO:0000281 ISS
    kidney development GO:0001822 IEA
    kidney development GO:0001822 ISO
    kidney development GO:0001822 ISS
    post-Golgi vesicle-mediated transport GO:0006892 ISO
    Golgi to plasma membrane transport GO:0006893 IEA
    Golgi to plasma membrane transport GO:0006893 ISO
    Golgi to plasma membrane transport GO:0006893 ISS
    smoothened signaling pathway GO:0007224 IEA
    smoothened signaling pathway GO:0007224 ISO
    small GTPase-mediated signal transduction GO:0007264 IEA
    small GTPase-mediated signal transduction GO:0007264 ISO
    small GTPase-mediated signal transduction GO:0007264 ISS
    protein transport GO:0015031 IEA
    intraciliary transport GO:0042073 IEA
    intraciliary transport GO:0042073 ISO
    photoreceptor cell development GO:0042461 IEA
    photoreceptor cell development GO:0042461 ISO
    photoreceptor cell development GO:0042461 ISS
    cell division GO:0051301 IEA
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IEA
    cilium assembly GO:0060271 ISO
    cilium assembly GO:0060271 ISS
    protein localization to cilium GO:0061512 ISO
    protein localization to ciliary membrane GO:1903441 IEA
    protein localization to ciliary membrane GO:1903441 ISO
    protein localization to ciliary membrane GO:1903441 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    spindle GO:0005819 IEA
    spindle microtubule GO:0005876 IEA
    spindle microtubule GO:0005876 ISO
    spindle microtubule GO:0005876 ISS
    cytoplasmic microtubule GO:0005881 IEA
    cytoplasmic microtubule GO:0005881 ISO
    cytoplasmic microtubule GO:0005881 ISS
    cilium GO:0005929 IEA
    cilium GO:0005929 ISO
    cilium GO:0005929 ISO
    cilium GO:0005929 ISS
    axoneme GO:0005930 IEA
    axoneme GO:0005930 ISO
    microtubule cytoskeleton GO:0015630 IBA
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    photoreceptor connecting cilium GO:0032391 IEA
    photoreceptor connecting cilium GO:0032391 ISO
    photoreceptor connecting cilium GO:0032391 ISS
    ciliary transition zone GO:0035869 ISO
    ciliary basal body GO:0036064 IEA
    ciliary basal body GO:0036064 ISO
 Experiment description of studies that identified Arl3 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Arl3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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