Gene description for Flot1
Gene name flotillin 1
Gene symbol Flot1
Other names/aliases Rareg
Species Rattus norvegicus
 Database cross references - Flot1
ExoCarta ExoCarta_64665
Vesiclepedia VP_64665
Entrez Gene 64665
UniProt Q9Z1E1  
 Flot1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Cortical neurones 16446100    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Flot1
Molecular Function
    protease binding GO:0002020 IBA
    protease binding GO:0002020 IPI
    protease binding GO:0002020 ISO
    protein binding GO:0005515 IPI
    ionotropic glutamate receptor binding GO:0035255 IPI
Biological Process
    positive regulation of cytokine production GO:0001819 ISO
    positive regulation of protein phosphorylation GO:0001934 ISO
    regulation of receptor internalization GO:0002090 IBA
    regulation of receptor internalization GO:0002090 ISO
    axonogenesis GO:0007409 ISO
    extracellular matrix disassembly GO:0022617 ISO
    positive regulation of synaptic transmission, dopaminergic GO:0032226 ISO
    positive regulation of interferon-beta production GO:0032728 ISO
    dsRNA transport GO:0033227 ISO
    positive regulation of heterotypic cell-cell adhesion GO:0034116 ISO
    positive regulation of toll-like receptor 3 signaling pathway GO:0034141 ISO
    regulation of toll-like receptor 4 signaling pathway GO:0034143 ISO
    response to endoplasmic reticulum stress GO:0034976 ISO
    regulation of Rho protein signal transduction GO:0035023 ISO
    intracellular signal transduction GO:0035556 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    plasma membrane raft assembly GO:0044854 ISO
    plasma membrane raft organization GO:0044857 ISO
    positive regulation of endocytosis GO:0045807 IBA
    positive regulation of endocytosis GO:0045807 ISO
    positive regulation of skeletal muscle tissue development GO:0048643 ISO
    protein stabilization GO:0050821 ISO
    regulation of neurotransmitter uptake GO:0051580 ISO
    protein kinase C signaling GO:0070528 IBA
    cellular response to exogenous dsRNA GO:0071360 ISO
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to plasma membrane GO:0072659 ISO
    positive regulation of myoblast fusion GO:1901741 ISO
    positive regulation of cell junction assembly GO:1901890 IBA
    positive regulation of cell junction assembly GO:1901890 ISO
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 IBA
    positive regulation of cell-cell adhesion mediated by cadherin GO:2000049 ISO
Subcellular Localization
    uropod GO:0001931 ISO
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    endosome GO:0005768 ISS
    early endosome GO:0005769 ISO
    microtubule organizing center GO:0005815 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    caveola GO:0005901 IDA
    caveola GO:0005901 ISO
    cell-cell junction GO:0005911 ISO
    adherens junction GO:0005912 ISO
    COP9 signalosome GO:0008180 ISO
    external side of plasma membrane GO:0009897 ISO
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 ISO
    flotillin complex GO:0016600 IBA
    flotillin complex GO:0016600 IDA
    flotillin complex GO:0016600 IEA
    flotillin complex GO:0016600 ISO
    lamellipodium GO:0030027 ISO
    cortical actin cytoskeleton GO:0030864 ISO
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 ISO
    centriolar satellite GO:0034451 ISO
    sarcolemma GO:0042383 ISO
    melanosome GO:0042470 IEA
    cell-cell contact zone GO:0044291 ISO
    plasma membrane raft GO:0044853 ISO
    membrane raft GO:0045121 ISO
    apical part of cell GO:0045177 ISO
    synapse GO:0045202 IDA
    presynaptic active zone GO:0048786 EXP
    presynaptic active zone GO:0048786 IDA
    dopaminergic synapse GO:0098691 ISO
    presynapse GO:0098793 IDA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    GABA-ergic synapse GO:0098982 EXP
    GABA-ergic synapse GO:0098982 IDA
 Experiment description of studies that identified Flot1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 30
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Flot1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here