Gene description for Ap3m2
Gene name adaptor-related protein complex 3, mu 2 subunit
Gene symbol Ap3m2
Other names/aliases 5830445E16Rik
AP-3B
Species Mus musculus
 Database cross references - Ap3m2
ExoCarta ExoCarta_64933
Vesiclepedia VP_64933
Entrez Gene 64933
UniProt Q8R2R9  
 Ap3m2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ap3m2
Biological Process
    intracellular protein transport GO:0006886 IEA
    endocytosis GO:0006897 IBA
    anterograde axonal transport GO:0008089 IMP
    synaptic vesicle coating GO:0016183 NAS
    clathrin-coated vesicle cargo loading, AP-3-mediated GO:0035654 NAS
    synaptic vesicle recycling GO:0036465 NAS
    intracellular transport GO:0046907 NAS
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    anterograde synaptic vesicle transport GO:0048490 IMP
Subcellular Localization
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 IEA
    synaptic vesicle GO:0008021 IEA
    AP-3 adaptor complex GO:0030123 NAS
    clathrin adaptor complex GO:0030131 IEA
    cytoplasmic vesicle membrane GO:0030659 IEA
    cytoplasmic vesicle GO:0031410 IBA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    GABA-ergic synapse GO:0098982 IDA
    GABA-ergic synapse GO:0098982 IMP
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Ap3m2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap3m2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snapin  
Co-fractionation Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ap3m2 is involved
No pathways found





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