Gene description for Arl2
Gene name ADP-ribosylation factor-like 2
Gene symbol Arl2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arl2
ExoCarta ExoCarta_65142
Entrez Gene 65142
UniProt O08697  
 Arl2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Arl2
Molecular Function
    GTP binding GO:0005525 ISO
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    GDP binding GO:0019003 ISO
Biological Process
    bicellular tight junction assembly GO:0070830 ISS
    metabolic process GO:0008152 ISS
    small GTPase mediated signal transduction GO:0007264 IEA
    negative regulation of GTPase activity GO:0034260 ISS
    centrosome organization GO:0051297 ISS
    positive regulation of cell-substrate adhesion GO:0010811 ISS
    acetylcholine transport GO:0015870 IDA
    maintenance of protein location in nucleus GO:0051457 ISS
    regulation of microtubule polymerization GO:0031113 ISS
    positive regulation of microtubule polymerization GO:0031116 ISS
Subcellular Localization
    nucleus GO:0005634 ISS
    cytosol GO:0005829 IDA
    extracellular exosome GO:0070062 ISO
    mitochondrial intermembrane space GO:0005758 ISO
    Golgi apparatus GO:0005794 IDA
    cytoplasm GO:0005737 IDA
    primary cilium GO:0072372 ISO
    intracellular GO:0005622 IEA
    centrosome GO:0005813 ISS
    lateral plasma membrane GO:0016328 ISS
 Experiment description of studies that identified Arl2 in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Arl2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Arl2 is involved
No pathways found





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