Gene description for Tmed2 |
Gene name |
transmembrane emp24 domain trafficking protein 2 |
Gene symbol |
Tmed2 |
Other names/aliases |
Rnp24 |
Species |
Rattus norvegicus |
Database cross references - Tmed2 |
ExoCarta |
ExoCarta_65165 |
Vesiclepedia |
VP_65165 |
Entrez Gene |
65165 |
UniProt |
Q63524
|
Tmed2 identified in exosomes derived from the following tissue/cell type |
Hepatocytes
|
19367702
|
Reticulocytes
|
21828046
|
Reticulocytes
|
21828046
|
Reticulocytes
|
21828046
|
Reticulocytes
|
21828046
|
Reticulocytes
|
21828046
|
Gene ontology annotations for Tmed2 |
|
Experiment description of studies that identified Tmed2 in exosomes |
1 |
Experiment ID |
35 |
MISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
TSG101|Alix|CD81|CD63
|
EV Enriched markers |
✔
HSPA5
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19367702
|
Organism |
Rattus norvegicus |
Experiment description |
Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. |
Authors |
Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM |
Journal name |
JPR
|
Publication year |
2008 |
Sample |
Hepatocytes |
Sample name |
Hepatocytes |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting |
|
|
2 |
Experiment ID |
90 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSC70|HSP90|TSG101|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D2 Rep 1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
3 |
Experiment ID |
94 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D4 Rep 1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
4 |
Experiment ID |
96 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D2 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
5 |
Experiment ID |
97 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D4 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
6 |
Experiment ID |
98 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D7 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.25 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
Protein-protein interactions for Tmed2 |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
No interactions are found.
|
|
Pathways in which Tmed2 is involved |
|
|
|