Gene description for Tmed2
Gene name transmembrane emp24 domain trafficking protein 2
Gene symbol Tmed2
Other names/aliases Rnp24
Species Rattus norvegicus
 Database cross references - Tmed2
ExoCarta ExoCarta_65165
Vesiclepedia VP_65165
Entrez Gene 65165
UniProt Q63524  
 Tmed2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tmed2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    heart looping GO:0001947 ISO
    negative regulation of GTPase activity GO:0034260 ISS
    in utero embryonic development GO:0001701 ISO
    vesicle targeting GO:0006903 NAS
    Golgi organization GO:0007030 ISS
    multicellular organism growth GO:0035264 ISO
    protein targeting to plasma membrane GO:0072661 ISS
    embryonic placenta development GO:0001892 ISO
    post-anal tail morphogenesis GO:0036342 ISO
    somite rostral/caudal axis specification GO:0032525 ISO
    intracellular protein transport GO:0006886 NAS
    neural tube closure GO:0001843 ISO
    positive regulation of gene expression GO:0010628 ISO
    somitogenesis GO:0001756 ISO
    maternal placenta development GO:0001893 IEA
    embryonic morphogenesis GO:0048598 IEA
Subcellular Localization
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
    COPI-coated vesicle GO:0030137 ISS
    COPI-coated vesicle membrane GO:0030663 IEA
    integral component of membrane GO:0016021 IDA
    Golgi apparatus GO:0005794 ISS
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISS
    endoplasmic reticulum GO:0005783 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    Golgi cisterna membrane GO:0032580 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    zymogen granule membrane GO:0042589 IDA
 Experiment description of studies that identified Tmed2 in exosomes
1
Experiment ID 35
MISEV standards
CEM
EV Biophysical techniques
TSG101|Alix|CD81|CD63
EV Enriched markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
MISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tmed2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tmed2 is involved
PathwayEvidenceSource
Pre-NOTCH Processing in Golgi IEA Reactome





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