Gene description for Limk1
Gene name LIM domain kinase 1
Gene symbol Limk1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Limk1
ExoCarta ExoCarta_65172
Vesiclepedia VP_65172
Entrez Gene 65172
 Limk1 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Limk1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IEA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IEA
    heat shock protein binding GO:0031072 IEA
    heat shock protein binding GO:0031072 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    actin cytoskeleton organization GO:0030036 IBA
    positive regulation of actin filament bundle assembly GO:0032233 ISO
    stress fiber assembly GO:0043149 IEA
    stress fiber assembly GO:0043149 ISO
    positive regulation of axon extension GO:0045773 IEA
    positive regulation of axon extension GO:0045773 ISO
    positive regulation of axon extension GO:0045773 ISS
    axon extension GO:0048675 IEA
    axon extension GO:0048675 ISO
    negative regulation of ubiquitin-protein transferase activity GO:0051444 ISO
    positive regulation of stress fiber assembly GO:0051496 IBA
    positive regulation of stress fiber assembly GO:0051496 IEA
    positive regulation of stress fiber assembly GO:0051496 ISO
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 IDA
    regulation of modification of postsynaptic actin cytoskeleton GO:1905274 IMP
Subcellular Localization
    male germ cell nucleus GO:0001673 IEA
    male germ cell nucleus GO:0001673 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 IEA
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    lamellipodium GO:0030027 ISS
    neuron projection GO:0043005 IBA
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    neuron projection GO:0043005 ISS
    axonal growth cone GO:0044295 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    postsynapse GO:0098794 IEA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Limk1 in sEVs
1
Experiment ID 92
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Limk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rnf6  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Limk1 is involved
No pathways found





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