Gene description for Limk1
Gene name LIM domain kinase 1
Gene symbol Limk1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Limk1
ExoCarta ExoCarta_65172
Entrez Gene 65172
 Limk1 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Limk1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IEA
    ATP binding GO:0005524 IEA
    heat shock protein binding GO:0031072 ISO
    protein heterodimerization activity GO:0046982 ISO
    protein kinase activity GO:0004672 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    protein phosphorylation GO:0006468 ISS
    positive regulation of axon extension GO:0045773 ISS
    positive regulation of stress fiber assembly GO:0051496 ISO
    positive regulation of actin filament bundle assembly GO:0032233 ISO
    negative regulation of ubiquitin-protein transferase activity GO:0051444 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    neuron projection GO:0043005 ISS
    axonal growth cone GO:0044295 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    focal adhesion GO:0005925 ISO
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 IDA
    nucleus GO:0005634 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Limk1 in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Limk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Limk1 is involved
No pathways found





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