Gene description for Rsad2
Gene name radical S-adenosyl methionine domain containing 2
Gene symbol Rsad2
Other names/aliases Best5
Vig1
Species Rattus norvegicus
 Database cross references - Rsad2
ExoCarta ExoCarta_65190
Entrez Gene 65190
UniProt O70600  
 Rsad2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rsad2
Molecular Function
    4 iron, 4 sulfur cluster binding GO:0051539 ISS
    protein self-association GO:0043621 ISS
    catalytic activity GO:0003824 IEA
    molecular_function GO:0003674 ND
    metal ion binding GO:0046872 IEA
Biological Process
    innate immune response GO:0045087 IEA
    positive regulation of toll-like receptor 7 signaling pathway GO:0034157 ISS
    negative regulation of viral genome replication GO:0045071 ISO
    regulation of ossification GO:0030278 IDA
    response to virus GO:0009615 ISS
    metabolic process GO:0008152 IEA
    positive regulation of T-helper 2 cell cytokine production GO:2000553 ISS
    negative regulation of protein secretion GO:0050709 ISS
    positive regulation of toll-like receptor 9 signaling pathway GO:0034165 ISS
    defense response to virus GO:0051607 ISS
    ossification GO:0001503 IDA
    CD4-positive, alpha-beta T cell activation GO:0035710 ISS
    CD4-positive, alpha-beta T cell differentiation GO:0043367 ISS
Subcellular Localization
    mitochondrial outer membrane GO:0005741 ISO
    mitochondrion GO:0005739 ISO
    lipid particle GO:0005811 ISS
    mitochondrial inner membrane GO:0005743 ISO
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 ISS
 Experiment description of studies that identified Rsad2 in exosomes
1
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rsad2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rsad2 is involved
No pathways found





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