Gene description for Kcnn4
Gene name potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4
Gene symbol Kcnn4
Other names/aliases KCa3.1
rKCNN4c
rSK4
Species Rattus norvegicus
 Database cross references - Kcnn4
ExoCarta ExoCarta_65206
Vesiclepedia VP_65206
Entrez Gene 65206
 Kcnn4 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Kcnn4
Molecular Function
    inward rectifier potassium channel activity GO:0005242 IDA
    potassium channel activity GO:0005267 ISO
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 IEA
    calmodulin binding GO:0005516 ISO
    calcium-activated potassium channel activity GO:0015269 ISO
    small conductance calcium-activated potassium channel activity GO:0016286 IEA
    protein phosphatase binding GO:0019903 IEA
    protein phosphatase binding GO:0019903 ISO
    intermediate conductance calcium-activated potassium channel activity GO:0022894 IBA
    intermediate conductance calcium-activated potassium channel activity GO:0022894 IDA
    intermediate conductance calcium-activated potassium channel activity GO:0022894 IEA
    intermediate conductance calcium-activated potassium channel activity GO:0022894 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
Biological Process
    response to hypoxia GO:0001666 IEP
    regulation of angiotensin levels in blood GO:0002002 IMP
    cardiac muscle hypertrophy GO:0003300 IMP
    potassium ion transport GO:0006813 IDA
    calcium ion transport GO:0006816 IEA
    calcium ion transport GO:0006816 ISO
    monoatomic anion transport GO:0006820 IDA
    cell volume homeostasis GO:0006884 IEA
    cell volume homeostasis GO:0006884 ISO
    negative regulation of cardiac muscle hypertrophy GO:0010614 IMP
    stabilization of membrane potential GO:0030322 IEA
    stabilization of membrane potential GO:0030322 ISO
    regulation of tumor necrosis factor production GO:0032680 IMP
    response to insulin GO:0032868 IEP
    macropinocytosis GO:0044351 IEA
    macropinocytosis GO:0044351 ISO
    phospholipid translocation GO:0045332 IEA
    phospholipid translocation GO:0045332 ISO
    negative regulation of cell volume GO:0045794 IMP
    saliva secretion GO:0046541 IEA
    saliva secretion GO:0046541 ISO
    regulation of smooth muscle cell proliferation GO:0048660 IMP
    positive regulation of smooth muscle cell proliferation GO:0048661 IMP
    positive regulation of protein secretion GO:0050714 IEA
    positive regulation of protein secretion GO:0050714 ISO
    positive regulation of T cell receptor signaling pathway GO:0050862 IEA
    positive regulation of T cell receptor signaling pathway GO:0050862 ISO
    protein homotetramerization GO:0051289 IEA
    protein homotetramerization GO:0051289 ISO
    establishment of localization in cell GO:0051649 IEA
    establishment of localization in cell GO:0051649 ISO
    positive regulation of G0 to G1 transition GO:0070318 IMP
    cellular response to hypoxia GO:0071456 IEP
    regulation of monocyte chemotactic protein-1 production GO:0071637 IMP
    mononuclear cell migration GO:0071674 IMP
    potassium ion transmembrane transport GO:0071805 IBA
    potassium ion transmembrane transport GO:0071805 IDA
    potassium ion transmembrane transport GO:0071805 IEA
    potassium ion transmembrane transport GO:0071805 ISO
    hepatocyte apoptotic process GO:0097284 IMP
    potassium ion export across plasma membrane GO:0097623 IDA
    regulation of renin secretion into blood stream GO:1900133 IMP
    positive regulation of p38MAPK cascade GO:1900745 IMP
    positive regulation of microglial cell activation GO:1903980 IMP
    vascular associated smooth muscle cell migration GO:1904738 IMP
    response to tetrachloromethane GO:1904772 IEP
    negative regulation of hepatic stellate cell proliferation GO:1904898 IMP
    regulation of calcium ion import across plasma membrane GO:1905664 IEA
    regulation of calcium ion import across plasma membrane GO:1905664 ISO
    vascular associated smooth muscle cell proliferation GO:1990874 IEP
Subcellular Localization
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 IEA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
    vesicle GO:0031982 IEA
    vesicle GO:0031982 ISO
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    neuron projection GO:0043005 IBA
    neuronal cell body GO:0043025 IBA
    neuronal cell body GO:0043025 IDA
 Experiment description of studies that identified Kcnn4 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Kcnn4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Kcnn4 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here