Gene description for Vti1a
Gene name vesicle transport through interaction with t-SNAREs 1A
Gene symbol Vti1a
Other names/aliases Vti1
Species Rattus norvegicus
 Database cross references - Vti1a
ExoCarta ExoCarta_65277
Vesiclepedia VP_65277
Entrez Gene 65277
UniProt Q9JI51  
 Vti1a identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Vti1a
Molecular Function
    SNARE binding GO:0000149 IBA
    SNAP receptor activity GO:0005484 IBA
    SNAP receptor activity GO:0005484 ISO
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IDA
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    Golgi to vacuole transport GO:0006896 IBA
    autophagy GO:0006914 ISO
    synaptic vesicle to endosome fusion GO:0016189 IDA
    synaptic vesicle to endosome fusion GO:0016189 IMP
    vesicle-mediated transport GO:0016192 IEA
    macroautophagy GO:0016236 IBA
    endocytic recycling GO:0032456 ISO
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    retrograde transport, endosome to Golgi GO:0042147 ISS
    vesicle fusion with Golgi apparatus GO:0048280 IBA
    vesicle fusion with Golgi apparatus GO:0048280 IDA
    voluntary musculoskeletal movement GO:0050882 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 IEA
    autophagosome GO:0005776 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    synaptic vesicle GO:0008021 IDA
    synaptic vesicle GO:0008021 IDA
    endomembrane system GO:0012505 IEA
    ER to Golgi transport vesicle membrane GO:0012507 IBA
    clathrin-coated vesicle GO:0030136 IDA
    clathrin-coated vesicle membrane GO:0030665 IEA
    synaptic vesicle membrane GO:0030672 IDA
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 IDA
    late endosome membrane GO:0031902 IBA
    neuronal cell body GO:0043025 IDA
    terminal bouton GO:0043195 HDA
    intracellular membrane-bounded organelle GO:0043231 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    neuron projection terminus GO:0044306 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    bounding membrane of organelle GO:0098588 IEA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IDA
 Experiment description of studies that identified Vti1a in exosomes
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Vti1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VAMP8 8673
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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