Gene description for SLC22A3
Gene name solute carrier family 22 (organic cation transporter), member 3
Gene symbol SLC22A3
Other names/aliases EMT
EMTH
OCT3
Species Homo sapiens
 Database cross references - SLC22A3
ExoCarta ExoCarta_6581
Vesiclepedia VP_6581
Entrez Gene 6581
HGNC 10967
MIM 604842
UniProt O75751  
 SLC22A3 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC22A3
Molecular Function
    protein binding GO:0005515 IPI
    quaternary ammonium group transmembrane transporter activity GO:0015651 IDA
    toxin transporter activity GO:0019534 IEA
    dopamine transmembrane transporter activity GO:0005329 IEA
    organic cation transmembrane transporter activity GO:0015101 IDA
Biological Process
    toxin transport GO:1901998 IEA
    quaternary ammonium group transport GO:0015697 IDA
    small molecule metabolic process GO:0044281 TAS
    dopamine transport GO:0015872 IEA
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    drug transmembrane transport GO:0006855 TAS
    transmembrane transport GO:0055085 TAS
    ammonium transmembrane transport GO:0072488 IEA
    regulation of appetite GO:0032098 IEA
    histamine uptake GO:0051615 IEA
    organic cation transport GO:0015695 IDA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    integral component of plasma membrane GO:0005887 TAS
 Experiment description of studies that identified SLC22A3 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC22A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SLC22A3 is involved
PathwayEvidenceSource
Abacavir transmembrane transport TAS Reactome
Organic cation transport TAS Reactome





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