Gene description for Eif3f
Gene name eukaryotic translation initiation factor 3, subunit F
Gene symbol Eif3f
Other names/aliases 0610037M02Rik
AA409853
Eif3s5
Species Mus musculus
 Database cross references - Eif3f
ExoCarta ExoCarta_66085
Entrez Gene 66085
UniProt Q9DCH4  
 Eif3f identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Mast cells 17486113    
Microglia 16081791    
 Gene ontology annotations for Eif3f
Molecular Function
    ubiquitin-specific protease activity GO:0004843 ISO
    translation initiation factor binding GO:0031369 IDA
    protein binding GO:0005515 IPI
    cysteine-type peptidase activity GO:0008234 IEA
    hydrolase activity GO:0016787 IEA
    translation initiation factor activity GO:0003743 ISO
    peptidase activity GO:0008233 IEA
Biological Process
    translation GO:0006412 IEA
    protein deubiquitination GO:0016579 ISO
    translational initiation GO:0006413 ISO
    proteolysis GO:0006508 IEA
Subcellular Localization
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IDA
    cytoplasm GO:0005737 IEA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Eif3f in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 14
ISEV standards
EM
EV Biophysical techniques
RAB7|RAB11
EV Cytosolic markers
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
DNM
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Eif3f
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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