Gene description for Ppih
Gene name peptidyl prolyl isomerase H
Gene symbol Ppih
Other names/aliases 1100001J08Rik
2010111B15Rik
4833408F11Rik
AI464484
CYP-20
CYPH
D4Wsu43e
Species Mus musculus
 Database cross references - Ppih
ExoCarta ExoCarta_66101
Vesiclepedia VP_66101
Entrez Gene 66101
UniProt Q9D868  
 Ppih identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppih
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISO
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 ISS
    cyclosporin A binding GO:0016018 IBA
    cyclosporin A binding GO:0016018 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
Biological Process
    protein peptidyl-prolyl isomerization GO:0000413 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    mRNA processing GO:0006397 IEA
    protein folding GO:0006457 IBA
    protein folding GO:0006457 IEA
    RNA splicing GO:0008380 IEA
    positive regulation of viral genome replication GO:0045070 ISO
Subcellular Localization
    spliceosomal complex GO:0005681 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    intracellular membrane-bounded organelle GO:0043231 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U4/U6 snRNP GO:0071001 ISO
 Experiment description of studies that identified Ppih in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppih
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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