Gene description for Pycrl
Gene name pyrroline-5-carboxylate reductase-like
Gene symbol Pycrl
Other names/aliases 1110058B13Rik
2700073G24Rik
Species Mus musculus
 Database cross references - Pycrl
ExoCarta ExoCarta_66194
Vesiclepedia VP_66194
Entrez Gene 66194
UniProt Q9DCC4  
 Pycrl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pycrl
Molecular Function
    cytidine deaminase activity GO:0004126 ISO
    pyrroline-5-carboxylate reductase activity GO:0004735 IBA
    pyrroline-5-carboxylate reductase activity GO:0004735 ISO
    pyrroline-5-carboxylate reductase activity GO:0004735 ISS
Biological Process
    cytidine deamination GO:0009972 ISO
    L-proline biosynthetic process GO:0055129 IBA
    L-proline biosynthetic process GO:0055129 IEA
    L-proline biosynthetic process GO:0055129 ISO
    L-proline biosynthetic process GO:0055129 ISS
Subcellular Localization
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
 Experiment description of studies that identified Pycrl in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pycrl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
2 Psmb1 19170
Co-fractionation Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Gldc  
Co-fractionation Mus musculus
5 Sdcbp 53378
Co-fractionation Mus musculus
6 Nudt16l1  
Co-fractionation Mus musculus
7 Fus  
Co-fractionation Mus musculus
8 Psmb5 19173
Co-fractionation Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Pycrl is involved
PathwayEvidenceSource
Glutamate and glutamine metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome





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