Gene description for Sec61b
Gene name Sec61 beta subunit
Gene symbol Sec61b
Other names/aliases 1190006C12Rik
AI326121
AW122942
Species Mus musculus
 Database cross references - Sec61b
ExoCarta ExoCarta_66212
Vesiclepedia VP_66212
Entrez Gene 66212
UniProt Q9CQS8  
 Sec61b identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Sec61b
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IDA
    ribosome binding GO:0043022 ISO
    epidermal growth factor binding GO:0048408 IEA
    epidermal growth factor binding GO:0048408 ISO
Biological Process
    SRP-dependent cotranslational protein targeting to membrane, translocation GO:0006616 IBA
    retrograde protein transport, ER to cytosol GO:0030970 IEA
    retrograde protein transport, ER to cytosol GO:0030970 ISO
    post-translational protein targeting to membrane, translocation GO:0031204 IBA
    ERAD pathway GO:0036503 ISO
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    Sec61 translocon complex GO:0005784 IBA
    Sec61 translocon complex GO:0005784 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    membrane GO:0016020 IBA
    membrane GO:0016020 ISO
    endoplasmic reticulum quality control compartment GO:0044322 IDA
    endoplasmic reticulum quality control compartment GO:0044322 ISO
 Experiment description of studies that identified Sec61b in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Sec61b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GLP1R  
PCA Homo sapiens
2 ASGR2  
Affinity Capture-Western Homo sapiens
3 Fancd2  
Affinity Capture-MS Mus musculus
4 Atg16l1  
Affinity Capture-MS Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Sec61b is involved
PathwayEvidenceSource
Insertion of tail-anchored proteins into the endoplasmic reticulum membrane IEA Reactome
Protein localization IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here