Gene description for Plb1
Gene name phospholipase B1
Gene symbol Plb1
Other names/aliases 4632413E21Rik
4930433E17Rik
4930539A06Rik
BC033606
Species Mus musculus
 Database cross references - Plb1
ExoCarta ExoCarta_665270
Vesiclepedia VP_665270
Entrez Gene 665270
UniProt Q3TTY0  
 Plb1 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Plb1
Molecular Function
    lysophospholipase activity GO:0004622 IBA
    lysophospholipase activity GO:0004622 IEA
    lysophospholipase activity GO:0004622 ISO
    phospholipase A2 activity GO:0004623 IBA
    phospholipase A2 activity GO:0004623 ISO
    triacylglycerol lipase activity GO:0004806 IEA
    triacylglycerol lipase activity GO:0004806 ISO
    calcium-dependent phospholipase A2 activity GO:0047498 IEA
    calcium-independent phospholipase A2 activity GO:0047499 IEA
    calcium-independent phospholipase A2 activity GO:0047499 ISO
    retinyl-palmitate esterase activity GO:0050253 IBA
    retinyl-palmitate esterase activity GO:0050253 IEA
    retinyl-palmitate esterase activity GO:0050253 ISO
Biological Process
    phospholipid metabolic process GO:0006644 IBA
    phospholipid metabolic process GO:0006644 ISO
    triglyceride catabolic process GO:0019433 IEA
    triglyceride catabolic process GO:0019433 ISO
    phosphatidylglycerol catabolic process GO:0034478 IEA
    phosphatidylglycerol catabolic process GO:0034478 ISO
    phosphatidylcholine catabolic process GO:0034638 IEA
    phosphatidylcholine catabolic process GO:0034638 ISO
    retinol metabolic process GO:0042572 IEA
    retinol metabolic process GO:0042572 ISO
    phosphatidylethanolamine catabolic process GO:0046338 IEA
    phosphatidylethanolamine catabolic process GO:0046338 ISO
    diacylglycerol catabolic process GO:0046340 IEA
    diacylglycerol catabolic process GO:0046340 ISO
    positive regulation of acrosome reaction GO:2000344 IMP
Subcellular Localization
    apical plasma membrane GO:0016324 IEA
    brush border membrane GO:0031526 IBA
    brush border membrane GO:0031526 IEA
    brush border membrane GO:0031526 ISO
 Experiment description of studies that identified Plb1 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Plb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Casz1  
Co-fractionation Mus musculus
View the network image/svg+xml



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