Gene description for Ddx18
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Gene symbol Ddx18
Other names/aliases 2310005B10Rik
Species Mus musculus
 Database cross references - Ddx18
ExoCarta ExoCarta_66942
Entrez Gene 66942
UniProt Q8K363  
 Ddx18 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ddx18
Molecular Function
    nucleic acid binding GO:0003676 IEA
    poly(A) RNA binding GO:0044822 ISO
    ATP binding GO:0005524 IEA
    RNA binding GO:0003723 IEA
    helicase activity GO:0004386 IEA
    hydrolase activity GO:0016787 IEA
    nucleotide binding GO:0000166 IEA
    ATP-dependent RNA helicase activity GO:0004004 IBA
Biological Process
    biological_process GO:0008150 ND
    RNA secondary structure unwinding GO:0010501 IBA
Subcellular Localization
    membrane GO:0016020 ISO
    nucleus GO:0005634 IEA
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Ddx18 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ddx18
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ddx18 is involved
No pathways found





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