Gene description for Slc38a2
Gene name solute carrier family 38, member 2
Gene symbol Slc38a2
Other names/aliases 5033402L14Rik
AI316867
ATA2
SAT2
mKIAA1382
Species Mus musculus
 Database cross references - Slc38a2
ExoCarta ExoCarta_67760
Vesiclepedia VP_67760
Entrez Gene 67760
UniProt Q8CFE6  
 Slc38a2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc38a2
Molecular Function
    amino acid:sodium symporter activity GO:0005283 ISO
    amino acid:sodium symporter activity GO:0005283 ISS
    neutral L-amino acid:sodium symporter activity GO:0005295 ISO
    neutral L-amino acid:sodium symporter activity GO:0005295 ISS
    proline:sodium symporter activity GO:0005298 ISO
    proline:sodium symporter activity GO:0005298 ISS
    amino acid transmembrane transporter activity GO:0015171 IDA
    amino acid transmembrane transporter activity GO:0015171 ISO
    acidic amino acid transmembrane transporter activity GO:0015172 ISO
    acidic amino acid transmembrane transporter activity GO:0015172 ISS
    neutral L-amino acid transmembrane transporter activity GO:0015175 IMP
    L-glutamine transmembrane transporter activity GO:0015186 IBA
    L-glutamine transmembrane transporter activity GO:0015186 IDA
    L-glutamine transmembrane transporter activity GO:0015186 ISO
    L-serine transmembrane transporter activity GO:0015194 IEA
    L-serine transmembrane transporter activity GO:0015194 ISO
    alanine:sodium symporter activity GO:0015655 ISO
    alanine:sodium symporter activity GO:0015655 ISS
Biological Process
    amino acid transmembrane transport GO:0003333 IBA
    amino acid transmembrane transport GO:0003333 ISO
    amino acid transport GO:0006865 IDA
    amino acid transport GO:0006865 ISO
    glutamine transport GO:0006868 IBA
    glutamine transport GO:0006868 IDA
    glutamine transport GO:0006868 ISO
    female pregnancy GO:0007565 IEA
    positive regulation of gene expression GO:0010628 ISO
    response to muscle activity GO:0014850 IEA
    acidic amino acid transport GO:0015800 IEA
    neutral amino acid transport GO:0015804 ISO
    proline transport GO:0015824 IMP
    L-serine transport GO:0015825 ISO
    cerebral cortex development GO:0021987 IEA
    glycine betaine transport GO:0031460 IEA
    glycine betaine transport GO:0031460 ISO
    alanine transport GO:0032328 ISO
    alanine transport GO:0032328 ISS
    positive regulation of RNA splicing GO:0033120 IEA
    positive regulation of RNA splicing GO:0033120 ISO
    cellular response to amino acid starvation GO:0034198 IEA
    sodium ion transmembrane transport GO:0035725 IEA
    amino acid import GO:0043090 ISO
    amino acid import GO:0043090 ISS
    cellular response to mechanical stimulus GO:0071260 IEA
    regulation of cellular response to stress GO:0080135 IEA
    regulation of cellular response to stress GO:0080135 ISO
    regulation of glutamate secretion, neurotransmission GO:1903294 ISO
    regulation of glutamate secretion, neurotransmission GO:1903294 ISS
    L-glutamine import across plasma membrane GO:1903803 ISO
    L-glutamine import across plasma membrane GO:1903803 ISS
    L-serine import across plasma membrane GO:1903812 ISO
    L-serine import across plasma membrane GO:1903812 ISS
    cellular response to arsenite(3-) GO:1903841 IEA
    cellular response to arsenite(3-) GO:1903841 ISO
    L-proline import across plasma membrane GO:1904271 ISO
    L-proline import across plasma membrane GO:1904271 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    brush border GO:0005903 IEA
    brush border GO:0005903 ISO
    axon GO:0030424 IEA
    axon GO:0030424 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    sarcolemma GO:0042383 IEA
    sarcolemma GO:0042383 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Slc38a2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc38a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Unk  
Affinity Capture-RNA Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here