Gene description for Arpc5
Gene name actin related protein 2/3 complex, subunit 5
Gene symbol Arpc5
Other names/aliases 5830443F10Rik
p16-Arc
Species Mus musculus
 Database cross references - Arpc5
ExoCarta ExoCarta_67771
Vesiclepedia VP_67771
Entrez Gene 67771
UniProt Q9CPW4  
 Arpc5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arpc5
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IEA
    actin filament binding GO:0051015 ISO
Biological Process
    smooth muscle cell migration GO:0014909 ISO
    cell migration GO:0016477 IBA
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    maintenance of cell polarity GO:0030011 ISO
    regulation of actin filament polymerization GO:0030833 IEA
    Arp2/3 complex-mediated actin nucleation GO:0034314 IBA
    Arp2/3 complex-mediated actin nucleation GO:0034314 IEA
    Arp2/3 complex-mediated actin nucleation GO:0034314 ISO
    actin filament network formation GO:0051639 ISO
    microtubule organizing center localization GO:0061842 ISO
    lamellipodium organization GO:0097581 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    endosome GO:0005768 ISO
    Arp2/3 protein complex GO:0005885 IBA
    Arp2/3 protein complex GO:0005885 ISO
    Arp2/3 protein complex GO:0005885 ISS
    Arp2/3 protein complex GO:0005885 TAS
    lamellipodium GO:0030027 IDA
    growth cone GO:0030426 ISO
    site of double-strand break GO:0035861 ISS
 Experiment description of studies that identified Arpc5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arpc5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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