Gene description for Ppa1
Gene name pyrophosphatase (inorganic) 1
Gene symbol Ppa1
Other names/aliases 2010317E03Rik
Pyp
Species Mus musculus
 Database cross references - Ppa1
ExoCarta ExoCarta_67895
Vesiclepedia VP_67895
Entrez Gene 67895
UniProt Q9D819  
 Ppa1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppa1
Molecular Function
    magnesium ion binding GO:0000287 IEA
    inorganic diphosphate phosphatase activity GO:0004427 IBA
    inorganic diphosphate phosphatase activity GO:0004427 IEA
    pyrophosphatase activity GO:0016462 IDA
Biological Process
    phosphate-containing compound metabolic process GO:0006796 IBA
    phosphate-containing compound metabolic process GO:0006796 IC
Subcellular Localization
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Ppa1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppa1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 Fitm2  
Positive Genetic Mus musculus
3 Nphs2  
Affinity Capture-MS Mus musculus
4 Sae1 56459
Co-fractionation Mus musculus
View the network image/svg+xml



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