Gene description for Hist1h1b
Gene name histone cluster 1, H1b
Gene symbol Hist1h1b
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Hist1h1b
ExoCarta ExoCarta_680522
Vesiclepedia VP_680522
Entrez Gene 680522
UniProt D3ZBN0  
 Hist1h1b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hist1h1b
Molecular Function
    DNA binding GO:0003677 ISO
    double-stranded DNA binding GO:0003690 IBA
    structural constituent of chromatin GO:0030527 IEA
    chromatin DNA binding GO:0031490 ISO
    nucleosomal DNA binding GO:0031492 IBA
    histone deacetylase binding GO:0042826 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    chromatin organization GO:0006325 ISO
    nucleosome assembly GO:0006334 IEA
    muscle organ development GO:0007517 ISO
    chromosome condensation GO:0030261 IBA
    negative regulation of DNA recombination GO:0045910 IBA
    protein stabilization GO:0050821 ISO
    establishment of protein localization to chromatin GO:0071169 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    nucleosome GO:0000786 IEA
    euchromatin GO:0000791 IBA
    heterochromatin GO:0000792 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    chromosome GO:0005694 ISO
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Hist1h1b in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hist1h1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
2 Snca  
Affinity Capture-MS Rattus norvegicus
3 Gja1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Hist1h1b is involved
PathwayEvidenceSource
Apoptosis IEA Reactome
Apoptosis induced DNA fragmentation IEA Reactome
Apoptotic execution phase IEA Reactome
Programmed Cell Death IEA Reactome





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