Gene description for Atp6v1a
Gene name ATPase, H+ transporting, lysosomal V1 subunit A
Gene symbol Atp6v1a
Other names/aliases Atp6v1a1
Species Rattus norvegicus
 Database cross references - Atp6v1a
ExoCarta ExoCarta_685232
Vesiclepedia VP_685232
Entrez Gene 685232
 Atp6v1a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Atp6v1a
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IEA
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IBA
    proton-transporting ATPase activity, rotational mechanism GO:0046961 ISO
Biological Process
    intracellular iron ion homeostasis GO:0006879 ISO
    proton motive force-driven ATP synthesis GO:0015986 IEA
    cellular response to increased oxygen levels GO:0036295 ISO
    ATP metabolic process GO:0046034 IEA
    synaptic vesicle lumen acidification GO:0097401 ISO
    proton transmembrane transport GO:1902600 IBA
Subcellular Localization
    vacuolar proton-transporting V-type ATPase, V1 domain GO:0000221 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 ISO
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 ISO
    secretory granule GO:0030141 ISO
    clathrin-coated vesicle membrane GO:0030665 IEA
    proton-transporting V-type ATPase complex GO:0033176 ISO
    proton-transporting V-type ATPase, V1 domain GO:0033180 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    extrinsic component of synaptic vesicle membrane GO:0098850 EXP
    extrinsic component of synaptic vesicle membrane GO:0098850 IDA
    ATPase complex GO:1904949 ISO
 Experiment description of studies that identified Atp6v1a in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atp6v1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Atp6v1a is involved
No pathways found





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