Gene description for Eif4a3
Gene name eukaryotic translation initiation factor 4A3
Gene symbol Eif4a3
Other names/aliases eIF4A-III
Species Rattus norvegicus
 Database cross references - Eif4a3
ExoCarta ExoCarta_688288
Entrez Gene 688288
UniProt Q3B8Q2  
 Eif4a3 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Eif4a3
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    ATP-dependent RNA helicase activity GO:0004004 ISO
    mRNA binding GO:0003729 ISO
    poly(A) binding GO:0008143 ISO
    ATP binding GO:0005524 ISO
Biological Process
    regulation of gene expression GO:0010468 IBA
    RNA splicing GO:0008380 IBA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 ISO
    rRNA processing GO:0006364 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    RNA secondary structure unwinding GO:0010501 IBA
    mRNA processing GO:0006397 IEA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 ISO
    negative regulation of translation GO:0017148 ISO
    embryonic cranial skeleton morphogenesis GO:0048701 ISS
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISS
    mRNA transport GO:0051028 IEA
    positive regulation of translation GO:0045727 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    nuclear speck GO:0016607 IEA
    exon-exon junction complex GO:0035145 ISO
    nucleus GO:0005634 ISO
    membrane GO:0016020 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Eif4a3 in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eif4a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here