Gene description for Prpf6
Gene name PRP6 pre-mRNA splicing factor 6 homolog (yeast)
Gene symbol Prpf6
Other names/aliases 1190003A07Rik
2610031L17Rik
ANT-1
U5-102K
Species Mus musculus
 Database cross references - Prpf6
ExoCarta ExoCarta_68879
Vesiclepedia VP_68879
Entrez Gene 68879
UniProt Q91YR7  
 Prpf6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prpf6
Molecular Function
    transcription coactivator activity GO:0003713 ISO
    RNA binding GO:0003723 ISO
    protein-macromolecule adaptor activity GO:0030674 ISO
    protein-macromolecule adaptor activity GO:0030674 ISS
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ribonucleoprotein complex binding GO:0043021 IEA
    ribonucleoprotein complex binding GO:0043021 ISO
    nuclear androgen receptor binding GO:0050681 ISO
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 IBA
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISS
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    RNA localization GO:0006403 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    U5 snRNP GO:0005682 IEA
    U5 snRNP GO:0005682 ISO
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IEA
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Prpf6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prpf6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Foxp3  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Mettl21e  
Affinity Capture-MS Mus musculus
5 Chaf1a  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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