Gene description for Hint1
Gene name histidine triad nucleotide binding protein 1
Gene symbol Hint1
Other names/aliases Pkci
Prkci
Species Rattus norvegicus
 Database cross references - Hint1
ExoCarta ExoCarta_690660
Vesiclepedia VP_690660
Entrez Gene 690660
UniProt P62959  
 Hint1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Hint1
Molecular Function
    nucleotide binding GO:0000166 IEA
    hydrolase activity GO:0016787 IBA
    hydrolase activity GO:0016787 ISO
    hydrolase activity GO:0016787 ISS
    deSUMOylase activity GO:0016929 ISO
    deSUMOylase activity GO:0016929 ISS
    adenosine 5'-monophosphoramidase activity GO:0043530 ISO
    adenosine 5'-monophosphoramidase activity GO:0043530 ISS
Biological Process
    regulation of DNA-templated transcription GO:0006355 ISO
    regulation of DNA-templated transcription GO:0006355 ISS
    proteolysis GO:0006508 IEA
    purine ribonucleotide catabolic process GO:0009154 IBA
    purine ribonucleotide catabolic process GO:0009154 ISO
    purine ribonucleotide catabolic process GO:0009154 ISS
    protein desumoylation GO:0016926 ISO
    protein desumoylation GO:0016926 ISS
    positive regulation of calcium-mediated signaling GO:0050850 ISO
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 ISO
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 ISS
Subcellular Localization
    histone deacetylase complex GO:0000118 ISO
    histone deacetylase complex GO:0000118 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Hint1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hint1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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