Gene description for Tmem30a
Gene name transmembrane protein 30A
Gene symbol Tmem30a
Other names/aliases 2010200I23Rik
AW540225
Cdc50a
D9Wsu20e
Species Mus musculus
 Database cross references - Tmem30a
ExoCarta ExoCarta_69981
Vesiclepedia VP_69981
Entrez Gene 69981
UniProt Q8VEK0  
 Tmem30a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tmem30a
Molecular Function
    structural molecule activity GO:0005198 IEA
    structural molecule activity GO:0005198 ISO
    protein binding GO:0005515 IPI
    aminophospholipid flippase activity GO:0015247 IEA
    aminophospholipid flippase activity GO:0015247 ISO
Biological Process
    xenobiotic transmembrane transport GO:0006855 IEA
    xenobiotic transmembrane transport GO:0006855 ISO
    positive regulation of neuron projection development GO:0010976 IDA
    aminophospholipid transport GO:0015917 ISO
    neuron projection development GO:0031175 ISO
    protein localization to endosome GO:0036010 IEA
    protein localization to endosome GO:0036010 ISO
    phospholipid translocation GO:0045332 IBA
    phospholipid translocation GO:0045332 IMP
    phospholipid translocation GO:0045332 ISO
    positive regulation of phospholipid translocation GO:0061092 IEA
    positive regulation of phospholipid translocation GO:0061092 ISO
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 IEA
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 ISO
    aminophospholipid translocation GO:0140331 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 IEA
    transport vesicle membrane GO:0030658 IEA
    early endosome membrane GO:0031901 IEA
    early endosome membrane GO:0031901 ISO
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
    phospholipid-translocating ATPase complex GO:1990531 ISO
    phospholipid-translocating ATPase complex GO:1990531 ISS
 Experiment description of studies that identified Tmem30a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tmem30a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Unk  
Affinity Capture-RNA Mus musculus
View the network image/svg+xml
 Pathways in which Tmem30a is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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