Gene description for Acot7
Gene name acyl-CoA thioesterase 7
Gene symbol Acot7
Other names/aliases 2410041A17Rik
Ach1
Act
Bach
CTE-IIa
Cte-II
Lach1
Species Mus musculus
 Database cross references - Acot7
ExoCarta ExoCarta_70025
Vesiclepedia VP_70025
Entrez Gene 70025
UniProt Q91V12  
 Acot7 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Acot7
Molecular Function
    fatty-acyl-CoA binding GO:0000062 ISO
    long-chain fatty acyl-CoA binding GO:0036042 ISO
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 ISO
    fatty acyl-CoA hydrolase activity GO:0047617 ISO
    carboxylic ester hydrolase activity GO:0052689 IEA
    long-chain fatty acyl-CoA hydrolase activity GO:0052816 IBA
    long-chain fatty acyl-CoA hydrolase activity GO:0052816 IDA
    long-chain fatty acyl-CoA hydrolase activity GO:0052816 ISO
Biological Process
    acyl-CoA metabolic process GO:0006637 IBA
    fatty acid catabolic process GO:0009062 IBA
    fatty acid catabolic process GO:0009062 IDA
    coenzyme A biosynthetic process GO:0015937 ISO
    medium-chain fatty-acyl-CoA catabolic process GO:0036114 ISO
    long-chain fatty-acyl-CoA catabolic process GO:0036116 ISO
    medium-chain fatty acid biosynthetic process GO:0051792 ISO
    palmitic acid biosynthetic process GO:1900535 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrial matrix GO:0005759 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IDA
 Experiment description of studies that identified Acot7 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Acot7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
2 Brca1  
Affinity Capture-Western Mus musculus
3 Tnfaip8  
Co-fractionation Mus musculus
4 Tia1  
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Acot7 is involved
PathwayEvidenceSource
Fatty acid metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Mitochondrial Fatty Acid Beta-Oxidation IEA Reactome





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