Gene description for Gorasp2
Gene name golgi reassembly stacking protein 2
Gene symbol Gorasp2
Other names/aliases 0610011A07Rik
2410043M02Rik
5730520M13Rik
9430094F20Rik
AW552058
GOLPH2
GRASP55
GRS2
p59
Species Mus musculus
 Database cross references - Gorasp2
ExoCarta ExoCarta_70231
Vesiclepedia VP_70231
Entrez Gene 70231
UniProt Q99JX3  
 Gorasp2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gorasp2
Molecular Function
    molecular_function GO:0003674 ND
Biological Process
    organelle organization GO:0006996 ISO
    organelle organization GO:0006996 ISS
    Golgi organization GO:0007030 IBA
    Golgi organization GO:0007030 ISO
    spermatogenesis GO:0007283 IEA
    cell differentiation GO:0030154 IEA
    response to endoplasmic reticulum stress GO:0034976 ISO
    response to endoplasmic reticulum stress GO:0034976 ISS
    establishment of protein localization to plasma membrane GO:0061951 ISO
    establishment of protein localization to plasma membrane GO:0061951 ISS
    organelle assembly GO:0070925 IEA
    organelle assembly GO:0070925 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    Golgi medial cisterna GO:0005797 ISO
 Experiment description of studies that identified Gorasp2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gorasp2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rpusd2  
Co-fractionation Mus musculus
2 Aamdc  
Co-fractionation Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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