Gene description for Plscr3
Gene name phospholipid scramblase 3
Gene symbol Plscr3
Other names/aliases 2210403O21Rik
2610037N06Rik
ESTM3
X83310
Species Mus musculus
 Database cross references - Plscr3
ExoCarta ExoCarta_70310
Vesiclepedia VP_70310
Entrez Gene 70310
UniProt Q9JIZ9  
 Plscr3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Plscr3
Molecular Function
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    SH3 domain binding GO:0017124 IEA
    phospholipid scramblase activity GO:0017128 IBA
    phospholipid scramblase activity GO:0017128 ISO
    phospholipid scramblase activity GO:0017128 ISS
    lead ion binding GO:0032791 ISO
    lead ion binding GO:0032791 ISS
    mercury ion binding GO:0045340 ISO
    mercury ion binding GO:0045340 ISS
    calcium-dependent protein binding GO:0048306 IEA
    calcium-dependent protein binding GO:0048306 ISO
Biological Process
    apoptotic process GO:0006915 IMP
    apoptotic process GO:0006915 ISO
    plasma membrane phospholipid scrambling GO:0017121 IBA
    plasma membrane phospholipid scrambling GO:0017121 IEA
    cardiolipin biosynthetic process GO:0032049 ISO
    cardiolipin biosynthetic process GO:0032049 ISS
    glucose homeostasis GO:0042593 IMP
    cholesterol homeostasis GO:0042632 IMP
    regulation of apoptotic process GO:0042981 ISO
    regulation of apoptotic process GO:0042981 ISS
    cellular response to lipopolysaccharide GO:0071222 IEP
    regulation of release of cytochrome c from mitochondria GO:0090199 ISO
    regulation of release of cytochrome c from mitochondria GO:0090199 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Plscr3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Plscr3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Plscr3 is involved
No pathways found





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