Experiment description of studies that identified TGM3 in exosomes

1 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 66 MISEV standards ✔ IEM Biophysical techniques ✔ TSG101|Alix|CD63|CD81 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

Protein-protein interactions for TGM3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 LIG4 3981 Affinity Capture-MS Homo sapiens 3 BPHL 670 Affinity Capture-MS Homo sapiens 4 INPP5A 3632 Affinity Capture-MS Homo sapiens 5 CCDC103   Affinity Capture-MS Homo sapiens 6 ORMDL3 94103 Affinity Capture-MS Homo sapiens 7 DYNC1I2 1781 Proximity Label-MS Homo sapiens 8 CDK15   Affinity Capture-MS Homo sapiens 9 ZBTB1   Affinity Capture-MS Homo sapiens 10 ZNF224   Affinity Capture-MS Homo sapiens 11 FNDC5   Affinity Capture-MS Homo sapiens 12 RHEB 6009 Affinity Capture-MS Homo sapiens 13 GSDMA 284110 Affinity Capture-MS Homo sapiens 14 B3GNT2 10678 Affinity Capture-MS Homo sapiens 15 MCM2 4171 Affinity Capture-MS Homo sapiens 16 E2F6 1876 Affinity Capture-MS Homo sapiens 17 FBXW7   Affinity Capture-MS Homo sapiens 18 GGCT 79017 Affinity Capture-MS Homo sapiens 19 FLG 2312 Affinity Capture-MS Homo sapiens 20 THAP10   Affinity Capture-MS Homo sapiens 21 KPRP 448834 Affinity Capture-MS Homo sapiens 22 UCHL5 51377 Reconstituted Complex Homo sapiens 23 PHF11 51131 Affinity Capture-MS Homo sapiens 24 IFNA4   Affinity Capture-MS Homo sapiens 25 AGPAT1 10554 Affinity Capture-MS Homo sapiens 26 NEDD4 4734 Affinity Capture-MS Homo sapiens 27 USP53 54532 Affinity Capture-MS Homo sapiens 28 KIF14 9928 Affinity Capture-MS Homo sapiens 29 NF2 4771 Affinity Capture-MS Homo sapiens 30 DSG1 1828 Affinity Capture-MS Homo sapiens 31 PARK2   Affinity Capture-MS Homo sapiens 32 ZDHHC19   Affinity Capture-MS Homo sapiens 33 RPS19BP1   Affinity Capture-MS Homo sapiens 34 MYCBPAP 84073 Affinity Capture-MS Homo sapiens 35 RNF38   Affinity Capture-MS Homo sapiens 36 ARHGEF40 55701 Affinity Capture-MS Homo sapiens 37 SRRT 51593 Affinity Capture-MS Homo sapiens 38 ASCL1   Affinity Capture-MS Homo sapiens 39 ARG1 383 Affinity Capture-MS Homo sapiens 40 PEMT   Affinity Capture-MS Homo sapiens 41 CCDC51 79714 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 FANCG 2189 Affinity Capture-MS Homo sapiens 43 TRIM21 6737 Affinity Capture-MS Homo sapiens 44 NTRK1 4914 Affinity Capture-MS Homo sapiens 45 PIGT 51604 Affinity Capture-MS Homo sapiens 46 SSUH2   Affinity Capture-MS Homo sapiens 47 ALDH3A1 218 Affinity Capture-MS Homo sapiens 48 APTX   Affinity Capture-MS Homo sapiens 49 DDX39B 7919 Affinity Capture-MS Homo sapiens 50 PRKAB1 5564 Affinity Capture-MS Homo sapiens 51 APIP 51074 Affinity Capture-MS Homo sapiens 52 SETD7 80854 Affinity Capture-MS Homo sapiens 53 CYLD   Affinity Capture-MS Homo sapiens 54 C1orf68 100129271 Affinity Capture-MS Homo sapiens 55 FBXL4 26235 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 56 CASP14 23581 Affinity Capture-MS Homo sapiens 57 RHBDD1 84236 Affinity Capture-MS Homo sapiens 58 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 59 GFER   Affinity Capture-MS Homo sapiens 60 FANCD2   Affinity Capture-MS Homo sapiens 61 VWA3B 200403 Affinity Capture-MS Homo sapiens 62 USP32P2   Affinity Capture-MS Homo sapiens 63 CCNC   Affinity Capture-MS Homo sapiens 64 DOCK8 81704 Affinity Capture-MS Homo sapiens 65 C16orf70   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 66 SIRT7   Affinity Capture-MS Homo sapiens 67 TBC1D22B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 MOGAT3   Affinity Capture-MS Homo sapiens 69 CD274 29126 Affinity Capture-MS Homo sapiens 70 ATP6AP2 10159 Affinity Capture-MS Homo sapiens 71 ZC3HC1   Affinity Capture-MS Homo sapiens 72 FTH1 2495 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 73 PLD5   Affinity Capture-MS Homo sapiens 74 CORO1C 23603 Affinity Capture-MS Homo sapiens 75 ADH1C 126 Affinity Capture-MS Homo sapiens 76 ANXA9   Affinity Capture-MS Homo sapiens 77 BLMH 642 Affinity Capture-MS Homo sapiens 78 SARAF   Affinity Capture-MS Homo sapiens 79 C18orf21   Affinity Capture-MS Homo sapiens 80 STX17 55014 Affinity Capture-MS Homo sapiens 81 PPME1 51400 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 82 DSP 1832 Affinity Capture-MS Homo sapiens 83 PRSS16 10279 Affinity Capture-MS Homo sapiens 84 TYMSOS   Affinity Capture-MS Homo sapiens 85 C21orf59 56683 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 86 SLX4   Affinity Capture-MS Homo sapiens 87 AMTN   Affinity Capture-MS Homo sapiens 88 TP53 7157 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 89 WWOX 51741 Affinity Capture-MS Homo sapiens 90 POLR2L 5441 Affinity Capture-MS Homo sapiens 91 MCM5 4174 Affinity Capture-MS Homo sapiens 92 PHF8   Affinity Capture-MS Homo sapiens 93 CAPZA2 830 Affinity Capture-MS Homo sapiens 94 DSC1 1823 Affinity Capture-MS Homo sapiens 95 C1orf35   Affinity Capture-MS Homo sapiens 96 PTDSS1 9791 Affinity Capture-MS Homo sapiens 97 PKP1 5317 Affinity Capture-MS Homo sapiens 98 WWP2 11060 Affinity Capture-MS Homo sapiens 99 MBNL1 4154 Affinity Capture-MS Homo sapiens 100 TRIM37   Proximity Label-MS Homo sapiens 101 DDX3X 1654 Affinity Capture-MS Homo sapiens 102 KIR3DS1   Affinity Capture-MS Homo sapiens 103 THAP4 51078 Affinity Capture-MS Homo sapiens 104 KDELR2 11014 Affinity Capture-MS Homo sapiens 105 SERPINB12 89777 Affinity Capture-MS Homo sapiens 106 PINK1   Affinity Capture-MS Homo sapiens 107 GOT1 2805 Affinity Capture-MS Homo sapiens 108 STK11 6794 Affinity Capture-MS Homo sapiens 109 ZUFSP 221302 Affinity Capture-MS Homo sapiens 110 ANXA2 302 Affinity Capture-MS Homo sapiens 111 CCR1   Affinity Capture-MS Homo sapiens 112 GUCY1A3   Affinity Capture-MS Homo sapiens 113 CSGALNACT1 55790 Affinity Capture-MS Homo sapiens 114 GALNT9   Affinity Capture-MS Homo sapiens 115 CUL4A 8451 Affinity Capture-MS Homo sapiens 116 FLG2 388698 Affinity Capture-MS Homo sapiens View the network

Pathways in which TGM3 is involved

No pathways found