Gene description for Prdm16
Gene name PR domain containing 16
Gene symbol Prdm16
Other names/aliases 5730557K01Rik
csp1
mel1
Species Mus musculus
 Database cross references - Prdm16
ExoCarta ExoCarta_70673
Entrez Gene 70673
UniProt A2A935  
 Prdm16 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Prdm16
Molecular Function
    sequence-specific DNA binding GO:0043565 ISO
    transcription coactivator activity GO:0003713 IDA
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
    SMAD binding GO:0046332 IPI
    metal ion binding GO:0046872 IEA
    nucleic acid binding GO:0003676 IEA
    activating transcription factor binding GO:0033613 ISO
Biological Process
    tongue development GO:0043586 IMP
    neurogenesis GO:0022008 IDA
    cell differentiation GO:0030154 IEA
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    palate development GO:0060021 IMP
    negative regulation of granulocyte differentiation GO:0030853 ISO
    transcription, DNA-templated GO:0006351 IEA
    white fat cell differentiation GO:0050872 IDA
    positive regulation of brown fat cell differentiation GO:0090336 IMP
    regulation of transcription, DNA-templated GO:0006355 IEA
    brown fat cell differentiation GO:0050873 IMP
    regulation of cellular respiration GO:0043457 IDA
    somatic stem cell maintenance GO:0035019 IDA
    positive regulation of transcription, DNA-templated GO:0045893 ISO
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IMP
    stem cell maintenance GO:0019827 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    transcriptional repressor complex GO:0017053 IDA
 Experiment description of studies that identified Prdm16 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Prdm16
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prdm16 is involved
PathwayEvidenceSource
PKMTs methylate histone lysines IEA Reactome





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