Gene description for Rfx4
Gene name regulatory factor X, 4 (influences HLA class II expression)
Gene symbol Rfx4
Other names/aliases 4933412G19Rik
NYD-sp10
Species Mus musculus
 Database cross references - Rfx4
ExoCarta ExoCarta_71137
Entrez Gene 71137
UniProt Q7TNK1  
 Rfx4 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rfx4
Molecular Function
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 ISO
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 IBA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 ISO
    chromatin binding GO:0003682 IDA
Biological Process
    negative regulation of smoothened signaling pathway involved in ventral spinal cord patterning GO:0021914 IMP
    cerebellar cortex morphogenesis GO:0021696 IMP
    forebrain development GO:0030900 IMP
    cilium assembly GO:0042384 IMP
    midbrain development GO:0030901 IMP
    telencephalon development GO:0021537 IMP
    regulation of transcription from RNA polymerase II promoter GO:0006357 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    transcription, DNA-templated GO:0006351 IEA
    regulation of transcription, DNA-templated GO:0006355 IBA
    transcription from RNA polymerase II promoter GO:0006366 ISO
    dorsal spinal cord development GO:0021516 IMP
    regulation of protein processing GO:0070613 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
 Experiment description of studies that identified Rfx4 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rfx4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rfx4 is involved
No pathways found





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