Gene description for Rfx4
Gene name regulatory factor X, 4 (influences HLA class II expression)
Gene symbol Rfx4
Other names/aliases 4933412G19Rik
NYD-sp10
Species Mus musculus
 Database cross references - Rfx4
ExoCarta ExoCarta_71137
Vesiclepedia VP_71137
Entrez Gene 71137
UniProt Q7TNK1  
 Rfx4 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rfx4
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IEA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IEA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO
    protein binding GO:0005515 IPI
    sequence-specific double-stranded DNA binding GO:1990837 ISO
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    telencephalon development GO:0021537 IMP
    negative regulation of smoothened signaling pathway GO:0045879 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    cilium assembly GO:0060271 IMP
    regulation of protein processing GO:0070613 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
 Experiment description of studies that identified Rfx4 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rfx4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ssbp3  
Two-hybrid Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
3 Usp7  
Affinity Capture-MS Mus musculus
4 Tcf3  
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Rfx4 is involved
No pathways found





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