Gene description for Rnf135
Gene name ring finger protein 135
Gene symbol Rnf135
Other names/aliases 0610037N03Rik
2410006N06Rik
Species Mus musculus
 Database cross references - Rnf135
ExoCarta ExoCarta_71956
Vesiclepedia VP_71956
Entrez Gene 71956
UniProt Q9CWS1  
 Rnf135 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rnf135
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin-protein transferase activity GO:0004842 ISO
    ubiquitin-protein transferase activity GO:0004842 ISS
    protein binding GO:0005515 IPI
    RIG-I binding GO:0039552 ISO
    RIG-I binding GO:0039552 ISS
    identical protein binding GO:0042802 ISO
    ribonucleoprotein complex binding GO:0043021 IBA
    ribonucleoprotein complex binding GO:0043021 ISO
    ribonucleoprotein complex binding GO:0043021 ISS
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin protein ligase activity GO:0061630 ISS
Biological Process
    protein polyubiquitination GO:0000209 IGI
    protein polyubiquitination GO:0000209 ISO
    free ubiquitin chain polymerization GO:0010994 ISO
    free ubiquitin chain polymerization GO:0010994 ISS
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISS
    positive regulation of interferon-beta production GO:0032728 ISO
    positive regulation of interferon-beta production GO:0032728 ISS
    RIG-I signaling pathway GO:0039529 IGI
    RIG-I signaling pathway GO:0039529 IMP
    RIG-I signaling pathway GO:0039529 ISO
    regulation of innate immune response GO:0045088 IBA
    regulation of innate immune response GO:0045088 ISO
    regulation of innate immune response GO:0045088 ISS
    protein homooligomerization GO:0051260 ISO
    protein homooligomerization GO:0051260 ISS
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K63-linked ubiquitination GO:0070534 ISS
    antiviral innate immune response GO:0140374 IMP
    antiviral innate immune response GO:0140374 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IC
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Rnf135 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rnf135
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Rnf135 is involved
No pathways found





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