Gene description for TRIO
Gene name trio Rho guanine nucleotide exchange factor
Gene symbol TRIO
Other names/aliases ARHGEF23
tgat
Species Homo sapiens
 Database cross references - TRIO
ExoCarta ExoCarta_7204
Entrez Gene 7204
HGNC 12303
MIM 601893
UniProt O75962  
 TRIO identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for TRIO
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IEA
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    enzyme binding GO:0019899 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    positive regulation of apoptotic process GO:0043065 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    transmembrane receptor protein tyrosine phosphatase signaling pathway GO:0007185 TAS
    apoptotic signaling pathway GO:0097190 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    axon guidance GO:0007411 TAS
    positive regulation of GTPase activity GO:0043547 TAS
    protein phosphorylation GO:0006468 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified TRIO in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRIO
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FLNA  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 PTPRF 5792
Affinity Capture-Western Homo sapiens
3 RAC1 5879
Reconstituted Complex Homo sapiens
4 BLMH 642
Two-hybrid Homo sapiens
5 RHOA 387
Invitro Homo sapiens
View the network image/svg+xml



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